Video Experimental Relacionado

Updated: Feb 28, 2026

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

Interacciones cooperativas y competitivas entre elementos regulatorios de la transcripción modulan la producción de

Yiyang Jin, Zhou Zhou, Jessica Qin Lin

bioRxiv : the preprint server for biology

|February 27, 2026

Resumen

Una nueva investigación revela cómo interactúan los elementos regulatorios de la transcripción (TRE), como los promotores y los potenciadores. Este estudio presenta CIERA-seq para mapear estas interacciones, mostrando que los promotores y los potenciadores reclutan factores específicos para modular la transcripción génica.

Área de la Ciencia:

Biología Molecular
Genética
Epigenética

Sus antecedentes:

La regulación de la transcripción depende de las interacciones entre diversos elementos de ADN.
Las especificidades de estas interacciones entre promotores y potenciadores no se comprenden completamente.
La comprensión de estas interacciones es crucial para descifrar el control de la expresión génica.

Objetivo del estudio:

Desarrollar un ensayo novedoso (CIERA-seq) para interrogar las interacciones promotor-potenciador.
Investigar la especificidad de las interacciones regulatorias entre elementos regulatorios de la transcripción (TRE) distales.
Elucidar los roles de los promotores y potenciadores en la modulación de la transcripción.

Principales métodos:

Desarrollo del Ensayo Reportero de Potenciadores Basado en Almohadillas de Aterrizaje Integrado en Cromatina (CIERA-seq).
Utilización de CIERA-seq para evaluar interacciones regulatorias en un contexto genómico controlado.
Análisis ortogonal utilizando datos de interferencia CRISPR (CRISPRi).

Principales resultados:

Ambos, promotores y potenciadores, exhiben actividades potenciadoras específicas sobre promotores diana.
Los TRE reclutan conjuntos distintos de factores: los promotores reclutan la maquinaria de transcripción central, los potenciadores reclutan factores pioneros y remodeladores.
Los promotores pueden ejercer efectos regulatorios negativos, potencialmente debido a la competencia por la maquinaria de transcripción, más que los potenciadores.

Conclusiones:

Los TRE funcionan como elementos cooperativos o competitivos dentro de los centros de transcripción.
Se propone un modelo en el que los TRE suministran componentes complementarios para superar los pasos limitantes de la velocidad en la transcripción.
Los hallazgos proporcionan un marco para comprender cómo los promotores y potenciadores regulan dinámicamente las producciones de transcripción.

Palabras clave:

interacciones cooperativas interacciones competitivas elementos regulatorios de la transcripción promotores potenciadores producción de transcripción CIERA-seq reclutamiento de factores de transcripción especificidad regulatoria

Más Videos Relacionados

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Videos de Conceptos Relacionados

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...

Co-activators and Co-repressors

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

Co-activators and Co-repressors

Co-activators and Co-repressors

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...