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Updated: Jun 28, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Protocolo para la formulación y evaluación de nanopartículas lipídicas a base de fitoesteroles como alternativas al

Jaewook Lee¹, Jae Hoon Kim¹, Seung-Yeul Lee¹

¹Genomictree Inc., Daejeon, Republic of Korea.

|February 27, 2026

Abstract:

We present a protocol for formulating and evaluating lipid nanoparticles (LNPs) that incorporate the plant-derived sterols ginsenoside Rg2 and protopanaxadiol (PPD) as substitutes for cholesterol. The protocol describes mRNA preparation, manual mRNA-LNP encapsulation, and physicochemical characterization. We further detail in vitro and in vivo procedures to evaluate mRNA delivery efficiency. This protocol provides a robust and cost-effective platform for efficient mRNA delivery. For complete details on the use and execution of this protocol, please refer to Park et al.¹.

Palabras clave:

Biotecnología e ingeniería biomédica Química Ciencia de materiales Organismos modelo

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