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Formulación y estabilización de fagos terapéuticos: una revisión

Eleni Siafakas¹, Hien T T Duong², Jonathan R Iredell³

¹Sydney Pharmacy School, The University of Sydney, New South Wales, Australia; Centre for Infectious Diseases and Microbiology, Westmead Institute for Medical Research, Sydney, New South Wales, Australia.

International journal of antimicrobial agents

|February 27, 2026

Abstract:

Bacteriophages (phages) have been revitalised as a promising alternative option to combat antibiotic resistant bacteria. However, the ability to upscale and commercialise this therapy is currently limited by the poor shelf-stability of therapeutic preparations. Phage therapy has been ongoing for a century yet a standardised approach to formulating therapeutic phages is currently lacking due to the diverse chemical landscape of these viruses and the different storage methods reported. With consideration of protein and biotherapeutic literature, this review examines the evidence surrounding the effects of container materials, buffers and media, salt and ions, pH, temperature, polymers, surfactants and semi-solid formulations on the shelf-stability of phages. Loss of viability appears to be phage dependent but can be attributed to surface adsorption, colloidal instability, aggregation phenomena and compromises to structural integrity and function. Thus, the aim of this review is to discuss current findings, identify knowledge gaps and inform the direction of future stabilisation studies.

Palabras clave:

Bacteriófagos coloides excipientes formulaciones no iónicas estabilidad de proteínas

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