Video Experimental Relacionado
Updated: Mar 1, 2026
07:22
Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
Published on: August 19, 2021
3.5K
Formulación y estabilización de fagos terapéuticos: una revisión
Eleni Siafakas1, Hien T T Duong2, Jonathan R Iredell3
1Sydney Pharmacy School, The University of Sydney, New South Wales, Australia; Centre for Infectious Diseases and Microbiology, Westmead Institute for Medical Research, Sydney, New South Wales, Australia.
International journal of antimicrobial agents
|February 27, 2026
Resumen
Los bacteriófagos (fagos) son una alternativa prometedora a los antibióticos, pero la escasa estabilidad en el almacenamiento dificulta su uso. Esta revisión explora los factores que afectan la viabilidad de los fagos, con el objetivo de mejorar la formulación y el almacenamiento para aplicaciones terapéuticas.
Área de la Ciencia:
- Microbiología
- Biotecnología
- Ciencias Farmacéuticas
Sus antecedentes:
- Los bacteriófagos (fagos) muestran potencial como alternativas a los antibióticos contra bacterias resistentes.
- Las limitaciones actuales en la comercialización de la terapia fágica provienen de la escasa estabilidad en el almacenamiento de las preparaciones de fagos.
- Existe una falta de enfoques de formulación estandarizados debido a la diversidad de fagos y a los variados métodos de almacenamiento.
Objetivo del estudio:
- Revisar la literatura existente sobre los factores que influyen en la estabilidad en el almacenamiento de fagos terapéuticos.
- Identificar lagunas de conocimiento en las estrategias de formulación y estabilización de fagos.
- Guiar la investigación futura hacia la mejora de la longevidad de la preparación de fagos.
Principales métodos:
- Revisión de literatura que integra datos de formulación de proteínas y bioterapias.
- Examen de los efectos de los materiales del contenedor, tampones, sales/iones, pH, temperatura, polímeros, tensioactivos y formulaciones semisólidas.
- Análisis de los mecanismos de pérdida de viabilidad del fago.
Principales resultados:
- La estabilidad en el almacenamiento depende del fago y está influenciada por varios parámetros de formulación y almacenamiento.
- Los mecanismos de pérdida de viabilidad incluyen adsorción superficial, inestabilidad coloidal, agregación y compromiso estructural/funcional.
- Diversos factores como el tipo de contenedor, el pH, la temperatura y los excipientes impactan significativamente la estabilidad del fago.
Conclusiones:
- Se necesitan protocolos de formulación estandarizados para mejorar la viabilidad clínica de la terapia fágica.
- Se requiere más investigación para optimizar la estabilización de fagos para uso terapéutico a gran escala.
- Abordar los desafíos de formulación es crucial para realizar el potencial completo de la terapia fágica.
Videos de Conceptos Relacionados
01:30Lytic Cycle of Bacteriophages
78.6K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
78.6K
01:26DNA Bacteriophages
1.2K
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.2K
00:43Lysogenic Cycle of Bacteriophages
68.3K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
68.3K
01:29Microorganisms in Medicine and Therapeutics
1.3K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.3K
01:16Viral Replication: Lysogenic Cycle
2.0K
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
2.0K
01:20Viral Replication: Lytic Cycle
2.0K
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
2.0K