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Cuantificación simultánea de alta sensibilidad de andrógenos y estrógenos clínicamente relevantes de alta potencia en

Patrick Caron¹, Michèle Rouleau¹, Chantal Guillemette²

¹Faculty of Pharmacy, Université Laval, Québec, QC, Canada; Université Laval Cancer Research Center (CRC), Québec, QC, Canada; Centre Hospitalier Universitaire de Québec Research Center (CHU de Québec) - Université Laval, Québec, QC, Canada.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

|February 28, 2026

Resumen

Un nuevo ensayo de cromatografía líquida-espectrometría de masas en tándem (LC-MS/MS) cuantifica 19 esteroides, incluidos andrógenos 11-oxigenados, en plasma humano. Este método de alta sensibilidad es crucial para la medición precisa de los niveles hormonales en entornos clínicos.

Sus antecedentes:

La cuantificación de hormonas esteroides es vital para diagnosticar y monitorizar diversos trastornos endocrinos.
Los ensayos existentes pueden carecer de la sensibilidad o amplitud necesarias para medir el espectro completo de esteroides relevantes, especialmente los novedosos o de baja abundancia.
Los andrógenos 11-oxigenados representan un área creciente de investigación con posible importancia clínica.

Conclusiones:

El ensayo LC-MS/MS desarrollado proporciona un método sensible y robusto para el perfilado completo de esteroides.
Su alta sensibilidad y amplia cobertura de analitos son críticas para una cuantificación precisa en diversos entornos clínicos.
El ensayo demostró aplicabilidad clínica en pacientes con cáncer de próstata, destacando su utilidad para monitorizar la terapia hormonal y en condiciones con bajas concentraciones de esteroides.

Palabras clave:

andrógenos 11-oxigenados muestras clínicas estrógenos LC-MS/MS espectrometría de masas esteroides

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