Protocolo para la preparación y caracterización de muestras para microsegundos combinados de congelación-hiperenfriamiento y espectroscopía de resonancia paramagnética electrónica

Microsecond freeze-hyperquenching (MHQ) coupled with pulsed electron-electron double resonance spectroscopy (PELDOR) can resolve conformational changes in biomolecules over space and time. Here, we present a protocol for preparing MHQ samples of a cyclic nucleotide-binding domain by rapid mixing with cyclic adenosine monophosphate. Further, we outline the acquisition and analysis of PELDOR data on MHQ samples and study the dynamics of protein-ligand interactions. This protocol can also be extended to other freeze-quench techniques beyond MHQ and other biomolecules. For complete details on the use and execution of this protocol, please refer to Hett et al.¹.