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Updated: Jul 31, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Plataforma de biosensores de flujo lateral de fluorescencia sin etiquetado basada en el reconocimiento
Zhaorui Song1, Xinhui Zhai2, Haihan Liu3
1Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai, Shandong 264117, China.
Abstract:
Rapid, accurate and convenient detection of illegally added drugs are of critical importance in food safety. Herein, based on the host-guest recognition strategy, we establish a novel label-free fluorescence lateral flow assay (LF-LFA) platform for detection of amantadine (AMD) in egg samples. Specially, two fluorescence reporters-BSA-β-CD@BER (formed via host-guest interaction) and BER-BSA (generated through AIE effect), are effectively designed. This platform can enable "turn-on" FL detection through host-guest interaction between cucurbit[7]uril (CB[7]) and AMD, as well as between CB[7] and BSA-β-CD@BER, without the need for fluorescent labeling of target molecule (AMD). As a result, it has successfully achieved sensitive detection of AMD with a low limit of detection (LOD = 2.1 ng mL-1), wide linear detection range (2.5-100 ng mL-1), high specificity (CR ≤ 4%) and fast response (only 8 min). It will be expected to provide a novel avenue for rapid on-site detection of more illegal drugs in practices.
