Variación asociada a la dieta en las comunidades bacterianas del intestino medio de larvas de Loxostege sticticalis alimentadas con plantas hospederas botánicamente distintas

Video Experimental Relacionado

Updated: Oct 14, 2026

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using ¹³C-Glucose

Published on: November 13, 2013

Dong Li¹, Jianyu Wang¹, Dandan Zhang¹

¹School of Grassland Science, Beijing Forestry University, Beijing, China.

Frontiers in insect science

|October 13, 2026

Abstracto

IntroduccióN:

La microbiota intestinal puede influir en la fisiología de los insectos y en sus interacciones ecológicas; sin embargo, aún no se comprende plenamente cómo las plantas hospedadoras botánicamente distintas afectan a las comunidades bacterianas intestinales en los insectos polífagos.

MéTodos:

Investigamos las comunidades bacterianas del intestino medio deLoxostege sticticalislarvas alimentadas con tres plantas hospederas de diferentes géneros o con una dieta artificial mediante secuenciación del gen 16S de ARNr. Se compararon la diversidad bacteriana, la composición de la comunidad, los taxa diferenciales y los perfiles funcionales predichos entre los tratamientos dietéticos.

Resultados:

Los tratamientos dietéticos se asociaron con diferencias en la estructura de la comunidad bacteriana intestinal. El grupo MS exhibió una mayor riqueza y diversidad bacteriana, mientras que el grupo CA mostró una comunidad bacteriana relativamente simplificada. El PCoA basado en Bray-Curtis y el PERMANOVA indicaron una variación general significativa en la composición de la comunidad entre los tratamientos dietéticos, aunque las pruebas exactas de PERMANOVA por pares no fueron significativas. Bacillota y Pseudomonadota fueron dominantes en todos los tratamientos, mientras que el grupo MS mostró una mayor representación de taxones asociados a Actinomycetota. El LEfSe identificó a los taxones relacionados con Actinomycetota como características distintivas del grupo MS y a los taxones relacionados con Bacillota como características del grupo AD. PICRUSt2 sugirió diferencias en el potencial funcional predicho entre los tratamientos dietéticos, incluyendo rutas relacionadas con el metabolismo de aminoácidos, el metabolismo de cofactores y vitaminas, y la biodegradación y metabolismo de xenobióticos.

DiscusióN:

Los resultados demuestran variaciones asociadas a la dieta en las comunidades bacterianas intestinales deL. sticticalisy proporcionan una base descriptiva para futuras investigaciones sobre las interacciones entre la dieta y la microbiota. Dado que el estudio incluyó solo tres réplicas biológicas agrupadas por tratamiento y no caracterizó la microbiota asociada a las plantas ni la química vegetal, las asociaciones observadas y las funciones predichas no deben interpretarse como evidencia de mecanismos microbianos específicos ni de adaptación del hospedador.

Palabras clave:

interacciones dieta-microbiota bacterias intestinales comunidades microbianas plaga polífaga funciones microbianas predichas

Más Videos Relacionados

Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle

Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle

Published on: October 8, 2021

Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome

Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome

Published on: March 31, 2023

Videos de Conceptos Relacionados

Microbiota of the Large Intestine

Microbiota of the Large Intestine

The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...

Microbiota of the Stomach and Small Intestine

Microbiota of the Stomach and Small Intestine

The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...

Bacterial Flora of the Large Intestine

Bacterial Flora of the Large Intestine

The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.

Development of Human Microbiota

Development of Human Microbiota

The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from the skin...

Dysbiosis of the Gut Microbiota

Dysbiosis of the Gut Microbiota

The human gut microbiome includes a diverse array of microbial species, including beneficial commensals and opportunistic pathogens, which interact to support host health. These microbes contribute to essential functions such as nutrient metabolism, immune system modulation, and maintenance of intestinal barrier integrity. However, disruptions to this equilibrium—referred to as dysbiosis—can have widespread physiological consequences.Dysbiosis is often characterized by reduced microbial...

Microbiota Modulation by Antibiotics

Microbiota Modulation by Antibiotics

Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...