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La secuencia intermedia en la región de codificación 26S del ARNr de T. thermophila se transcribe dentro del mayor

N Din, J Engberg, W Kaffenberger

|October 1, 1979

Resumen

La secuencia intermedia en T. thermophila.

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética La genética.
Biología celular Biología celular.

Sus antecedentes:

El ADN ribosomal de Tetrahymena thermophila (ADNr) contiene una secuencia intermedia dentro de la región de codificación del ARNr 26S.
Comprender la transcripción y el procesamiento de esta secuencia intermedia es crucial para comprender la expresión y regulación de genes de ARNr.

Objetivo del estudio:

Para investigar la transcripción de la secuencia intermedia en la región codificante de 26S rRNA de T. thermophila.
Para determinar cuándo se elimina la secuencia intermedia durante el procesamiento del ARNr.

Principales métodos:

Hibridación de precursores de ARNr y de ARNr maduro a fragmentos de ADNr.
Microscopía electrónica de los híbridos de bucle R.
Análisis de fragmentos de ADNr protegidos por S1 utilizando electroforesis en gel de agarosa alcalina.

Principales resultados:

El ARNr 26S maduro, el ARNr pre-26S y algunas moléculas de ARNr pre-maduro carecen de la secuencia intermedia.
Una fracción de las moléculas de pre-ARNr se hibridan con toda la región codificante, lo que indica que contienen la secuencia intermedia.
La secuencia intermedia se transcribe en la transcripción primaria de ADNr.

Conclusiones:

La secuencia intermedia se transcribe como parte de la transcripción primaria de ADNr en T. thermophila.
La eliminación post-transcripcional de la secuencia intermedia es un evento de procesamiento temprano en la maduración del ARNr de T. thermophila.

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