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Efecto reversible de la naloxona de los opioides en la pinocitosis en Amoeba proteus
Nature
|November 1, 1979
Resumen
Los opiáceos como la beta-endorfina suprimen la pinocitosis en Amoeba proteus al afectar el calcio. Este efecto es revertido por la naloxona, el aumento de sodio o el calcio añadido, revelando la influencia de los opioides en los procesos celulares.
Área de la Ciencia:
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
- Farmacología Farmacología.
Sus antecedentes:
- La pinocitosis en Amoeba proteus implica invaginación de la membrana y requiere calcio (Ca2+).
- La pinocitosis inducida por cationes está modulada por los niveles extracelulares de Ca2+ y el metabolismo celular del calcio.
- Se sabe que los opiáceos y los péptidos opioides afectan las membranas celulares y la dinámica del calcio.
Objetivo del estudio:
- Para investigar el efecto de los péptidos opioides y opiáceos específicos en la pinocitosis inducida por cationes en Amoeba proteus.
- Determinar el papel de los receptores de calcio y opiáceos en la mediación de estos efectos.
Principales métodos:
- Estudiar la pinocitosis inducida en el proteus de Amoeba utilizando sodio (Na+) o Tris+ como cationes.
- La administración de beta-endorfina, Met-enkefalina y morfina para observar los efectos sobre la pinocitosis.
- Utilizando el antagonista de los receptores de opiáceos (-) naloxona, variando las concentraciones de Na+ y añadiendo Ca2+ a los mecanismos de prueba.
Principales resultados:
- La beta-endorfina, la metenkefalina y la morfina suprimieron significativamente la pinocitosis inducida por Na+ o Tris+.
- Estos efectos supresores fueron revertidos o disminuidos por (-) naloxona, aumento de la concentración de Na +, o la adición de Ca2 +.
- Los hallazgos indican un mecanismo dependiente del calcio que involucra a los receptores de opiáceos.
Conclusiones:
- Los péptidos y opiáceos opioides inhiben la pinocitosis inducida por el catión en Amoeba proteus.
- Esta inhibición es mediada a través de los receptores opiáceos y depende de los niveles celulares de calcio.
- El estudio destaca una nueva interacción entre los opioides y los procesos celulares fundamentales como la pinocitosis.
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