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Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
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Video Experimental Relacionado

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Measuring the 50% Haemolytic Complement CH50 Activity of Serum
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Componente C3 del complemento secretado por líneas celulares establecidas.

D R Senger, R O Hynes

    Cell
    |October 1, 1978
    PubMed
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    Este resumen es generado por máquina.

    Las células de hámster secretan el complemento C3, que se activa a través de vías clásicas y alternativas. Este estudio revela que el C3 se sintetiza como un precursor inactivo (proC3) y se divide para convertirse en activo.

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    Área de la Ciencia:

    • Inmunología Inmunología.
    • Biología celular Biología celular.
    • La bioquímica es la bioquímica.

    Sus antecedentes:

    • La línea celular del hámster NIL8 secreta el componente 3 (C3), el colágeno y la fibronectina del complemento.
    • El C3 secretado existe como un complejo enlazado con disulfuro de polipéptidos alfa (130 kDa) y beta (65 kDa).

    Objetivo del estudio:

    • Investigar la síntesis, la activación y la participación en la vía del complemento de C3 secretado por las células NIL8.
    • Para caracterizar la forma precursora de C3 y su conversión al componente activo.

    Principales métodos:

    • Cultivo de células de hámster NIL8 y análisis de proteínas secretadas.
    • Investigando la activación de C3 utilizando zimosan y eritrocitos recubiertos de anticuerpos.
    • Caracterizar la forma precursora del C3 (proC3) y sus productos de escisión.

    Principales resultados:

    • Las células NIL8 secretan C3 que puede ser escindido a C3b y procesado por inactivadores de C3.
    • La activación de C3 a C3b está mediada por las vías clásicas y alternativas del complemento.
    • El C3 se sintetiza como un precursor inactivo de 185 kDa (proC3) que es escindido para producir C3 activo.

    Conclusiones:

    • El sistema de cultivo celular NIL8 proporciona un modelo para estudiar la síntesis y activación del complemento C3.
    • La secreción y activación de C3 se observan en algunas líneas celulares (NIL1, BALB/c 3T3) pero no en otras.
    • Los hallazgos aclaran el procesamiento biológico del complemento C3 de un precursor inactivo a un componente activo involucrado en las respuestas inmunes.