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RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Examination of the Telomere G-overhang Structure in Trypanosoma brucei

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Published on: January 27, 2011

Los ARNm del tripanosoma comparten una secuencia líder empalmada de 5' común.

M Parsons, R G Nelson, K P Watkins

|August 1, 1984

Resumen

Una secuencia de líder de 35 nucleótidos conservada se encuentra en la mayoría de los ARN mensajeros del tripanosoma (ARNm). Esta secuencia líder juega un papel en la regulación de la expresión génica en diferentes etapas de la vida.

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Área de la Ciencia:

Biología Molecular Biología Molecular
Parasitología Parasitología.
Genética La genética.

Sus antecedentes:

Los tripanosomas poseen mecanismos únicos de procesamiento de ARN.
Anteriormente se identificó una secuencia líder de 5' en ARNm trypanosome específicos.

Objetivo del estudio:

Investigar la prevalencia y las características de la secuencia 5' líder en los ARNm tripanosómicos.
Para entender el potencial regulatorio de esta secuencia de líderes.

Principales métodos:

Análisis de las bibliotecas de ADNc.
Caracterización de las transcripciones de ARN que contienen líder.

Principales resultados:

Una secuencia líder de 35 nucleótidos está presente en la mayoría de los ARNm del tripanosoma.
Las transcripciones que contienen liderazgo exhiben diversos patrones de expresión (específicos de la etapa, regulados, constitutivos).
No se encontró ningún vínculo directo entre las secuencias de líderes genómicos y los exones de genes.

Conclusiones:

La secuencia 5' líder es una característica común de los ARNm tripanosómicos.
Esta secuencia líder probablemente contribuye a la compleja regulación de la expresión génica en los tripanosomas.
Se necesita más investigación para dilucidar los mecanismos de la adición de la secuencia de líderes.