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El virus de la estomatitis vesicular infecta y madura sólo a través de la superficie basolateral de la línea celular
Cell
|August 1, 1984
Resumen
Las células del riñón canino Madin-Darby revelan infecciones virales distintas y polaridades en ciernes. El virus de la estomatitis vesicular (VSV) infecta y brota preferentemente desde el lado basolateral, mientras que el virus de la peste aviar (FPV) muestra menos polaridad.
Área de la Ciencia:
- Biología celular Biología celular.
- Virología Virología.
- Biología celular epitelial Biología celular epitelial
Sus antecedentes:
- Las células epiteliales polarizadas exhiben distintos dominios de la membrana apical y basolateral.
- La comprensión de la entrada del virus y el ensamblaje en las células polarizadas es crucial para la patogénesis y el control viral.
Objetivo del estudio:
- Investigar la polaridad de la infección y la maduración para el virus de la estomatitis vesicular (VSV) y el virus de la peste aviar (FPV) en las monocapas de células del riñón canino Madin-Darby (MDCK).
- Para determinar el sitio preferido de entrada viral y brotación en un modelo de epitelio polarizado.
Principales métodos:
- Utilizó células del riñón canino Madin-Darby (MDCK) cultivadas en filtros de nitrocelulosa para formar monocapas epiteliales polarizadas.
- Evaluación de la eficacia de la infección viral mediante la aplicación de VSV y FPV a las superficies apical y basolateral.
- Polaridad de brote viral cuantificada midiendo la liberación viral de ambos lados de la monocapa celular.
Principales resultados:
- Las células MDCK formaron monocapas epiteliales altamente resistentes.
- VSV infectó la superficie basolateral ~ 100 veces más eficientemente que la superficie apical.
- Más del 98% del VSV germinado se liberó desde el lado basal, mientras que >99% de la hemaglutinina FPV se liberó por vía apical.
- La polaridad del brote y la expresión de proteínas superficiales se mantuvieron durante al menos 10 horas.
Conclusiones:
- El VSV exhibe una fuerte polaridad tanto en la infección como en la maduración, utilizando preferentemente la membrana basolateral.
- FPV demuestra menos polaridad distinta en la infección, pero los brotes son predominantemente apical.
- Las monocapas de células MDCK sirven como un modelo valioso para estudiar las interacciones y la polaridad del virus y el epitelio.
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