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Biosíntesis de glicoproteínas en dictyostelium discoideum: regulación del desarrollo de los glicanos ligados a las

R L Ivatt, O P Das, E J Henderson

|September 1, 1984

Resumen

Dictyostelium discoideum El procesamiento de la glicoproteína cambia con la etapa de desarrollo. Las primeras etapas agregan fucosa, sulfato y fosfato, mientras que las últimas etapas recortan y agregan fucosa, influenciadas por la señalización celular.

Área de la Ciencia:

Biología celular y molecular Biología celular y molecular
Biología del desarrollo Biología del desarrollo.
Glucobiología Glucobiología.

Sus antecedentes:

La biosíntesis de oligosacáridos ligados a la glicoproteína N en Dictyostelium discoideum comienza con un glicano precursor transferido desde un portador de lípidos.
El procesamiento de oligosacáridos depende en gran medida de la etapa de desarrollo del organismo.

Objetivo del estudio:

Investigar las distintas vías de procesamiento de la glicoproteína durante el desarrollo temprano y tardío en D. discoideum.
Determinar el papel de los contactos intercelulares y la señalización cíclica de AMP (cAMP) en la regulación de estas vías de procesamiento.

Principales métodos:

Análisis del procesamiento de la glicoproteína en las células de D. discoideum en diferentes etapas de desarrollo (temprano vs. tarde).
Manipulación experimental de células (hambruna en suspensión agitada) para imitar señales de desarrollo.
Observación de cambios en las estructuras de los glicanos, incluidas las adiciones de fucosa, sulfato y fosfato, y el recorte de los glicanos precursores.

Principales resultados:

El procesamiento de desarrollo temprano retiene estructuras altas de mannosa, con modificaciones como adiciones de fucosa, sulfato y fosfato.
Estas reacciones de procesamiento temprano se pierden con la agregación celular.
El procesamiento de desarrollo tardío recorta ampliamente el precursor y agrega fucosa a las unidades periféricas de mannosa.
Estas modificaciones de etapa tardía coinciden con la formación de las puntas de los montículos celulares.

Conclusiones:

Dos conjuntos distintos de enzimas de procesamiento de glicoproteínas se expresan secuencialmente durante el desarrollo de D. discoideum.
Los contactos intercelulares y la señalización cAMP parecen suficientes para desencadenar la expresión controlada de estas vías de procesamiento alternativas.
La etapa de desarrollo y la señalización celular son reguladores críticos del procesamiento de oligosacáridos ligados a la glicoproteína N.

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