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El crecimiento autocrino inducido por oncogenes relacionados con el SRC en células mieloides transformadas de pollo
Cell
|December 1, 1984
Resumen
Los oncogenes virales de la familia de genes src permiten que las células mielóides de pollo produzcan el factor de crecimiento mielomonocítico de pollo (cMGF) de forma independiente. Este descubrimiento revela un mecanismo de crecimiento autocrino en la proliferación de células mieloides.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- Oncología Oncología.
Sus antecedentes:
- Los virus de la leucemia mieloide de pollo E26 y OK 10 transforman las células mieloides, haciéndolas dependientes del factor de crecimiento mielomonocítico de pollo (cMGF) para la proliferación.
- Los oncogenes virales están implicados en la transformación celular y el crecimiento incontrolado.
Objetivo del estudio:
- Investigar si los oncogenes de la familia de genes src pueden alterar la dependencia del factor de crecimiento de las células mieloides de pollo transformadas.
- Determinar si estos oncogenes inducen la producción autocrina de factores de crecimiento.
Principales métodos:
- Superinfección de las células transformadas E26 y OK 10 con retrovirus de la familia src.
- Análisis de la proliferación celular en ausencia de cMGF. exógeno.
- Caracterización de la actividad estimulante del crecimiento en medios condicionados utilizando ensayos biológicos e inmunológicos, incluida la neutralización de anticuerpos.
Principales resultados:
- Las células mieloides superinfectadas proliferaron sin cMGF exógeno.
- Medios acondicionados de células superinfectadas exhibieron actividad estimulante del crecimiento similar al cMGF.
- Esta actividad se redujo significativamente con el antisuero contra el cMGF, y la incubación de anticuerpos inhibió la proliferación celular.
Conclusiones:
- Los oncogenes virales de la familia Src inducen a las células mielóides de pollo a producir un factor similar al cMGF.
- Esta producción ocurre de manera autocrina, promoviendo la proliferación celular.
- Demuestra un mecanismo por el cual los oncogenes virales pueden conferir la independencia del factor de crecimiento.
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