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Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Published on: February 24, 2014
Las líneas celulares derivadas de tumores humanos contienen genes transformadores comunes y diferentes
M Perucho1, M Goldfarb, K Shimizu
1Cold Spring Harbor Laboratory, New York 11724.
Cell
|December 1, 1981
Resumen
El ADN de las células tumorales humanas puede transformar las células de ratón, revelando al menos tres genes distintos que conducen al cáncer. Estos hallazgos sugieren orígenes independientes para la superposición de vías que causan cáncer, avanzando en la investigación de la oncogénesis.
Área de la Ciencia:
- Oncología Oncología.
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- Las líneas de células tumorales humanas cultivadas son modelos cruciales para estudiar el desarrollo del cáncer.
- La identificación de los genes responsables de la transformación celular es clave para comprender la oncogénesis.
Objetivo del estudio:
- Para examinar las líneas celulares tumorales humanas en busca de ADN capaz de inducir la transformación en células de ratón NIH / 3T3.
- Identificar y caracterizar los genes específicos de transformación humana presentes en estas líneas tumorales.
Principales métodos:
- Cribado de ADN de la línea de células tumorales humanas para la inducción de transformación en células NIH / 3T3.
- Restricción de la digestión de la endonucleasa para analizar fragmentos de ADN.
- Detección de secuencias de ADN humano en células de ratón transformadas para confirmar el origen genético humano.
Principales resultados:
- Cinco líneas de células tumorales humanas demostraron la capacidad de transformar las células NIH / 3T3.
- Se identificaron al menos tres genes transformadores humanos distintos en estas cinco líneas celulares.
- Un subconjunto de tres líneas celulares (dos pulmonares, un carcinoma de colon) transfirió genes transformadores idénticos o estrechamente relacionados.
Conclusiones:
- El ADN tumoral humano contiene genes capaces de inducir la transformación celular en una especie diferente.
- La presencia de múltiples genes transformadores distintos sugiere diversos eventos genéticos en el cáncer.
- Las vías superpuestas en la tumorigénesis pueden surgir de forma independiente, ofreciendo información sobre la heterogeneidad del cáncer.
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