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Las mutaciones reguladoras en las células CHO inducen la expresión del antígeno embrionario SSEA-1 del ratón

C Campbell, P Stanley

|November 1, 1983

Resumen

Dos células mutantes del ovario del hámster chino (CHO), LEC11 y LEC12, expresan el antígeno embrionario del ratón SSEA-1 debido a las mutaciones reguladoras que afectan a los genes de la fucosiltransferasa. Estos mutantes muestran actividades enzimáticas distintas que no están presentes en las células parentales o revertentes.

Área de la Ciencia:

Biología celular Biología celular.
La bioquímica es la bioquímica.
Genética La genética.

Sus antecedentes:

Las células del ovario del hámster chino (CHO) son un sistema modelo común en la investigación biológica.
El antígeno embrionario del ratón SSEA-1 es un marcador clave del desarrollo.
Comprender las mutaciones en las líneas celulares ayuda a descifrar la regulación génica.

Objetivo del estudio:

Para investigar la base molecular de la expresión de SSEA-1 en las células CHO mutantes.
Para identificar las actividades enzimáticas específicas asociadas con la expresión de SSEA-1.
Para explorar la regulación genética de los genes de la fucosiltransferasa.

Principales métodos:

Se utilizó el radioinmunoensayo con anticuerpos monoclonales para detectar SSEA-1.
Se analizaron las actividades de la N-acetilglucosaminida alfa ((1,3) fucosiltransferasa en extractos celulares.
Comparación de las especificidades del sustrato de la enzima y los productos entre mutantes y células parentales.

Principales resultados:

Los mutantes de células CHO LEC11 y LEC12 expresan el antígeno SSEA-1.
Las células CHO parentales y los revertentes (LEC11.R9, LEC12.R10) no expresan SSEA-1.
La expresión de SSEA-1 se correlaciona con la actividad de novo de las N-acetilglucosaminidas alfa (1,3) fucosiltransferasas específicas.
Se observaron diferencias en las especificidades del sustrato de la enzima y en los productos.

Conclusiones:

Es probable que los mutantes LEC11 y LEC12 surjan de mutaciones regulatorias.
Estas mutaciones afectan a distintos genes de la fucosiltransferasa.
El estudio proporciona información sobre el control genético de la expresión y la fucosilación de SSEA-1 en las células CHO.

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