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Un anticuerpo monoclonal para la purificación a gran escala de los leucocitos humanos de interferón

D S Secher, D C Burke

|June 12, 1980

Resumen

Los investigadores aislaron un clon de mieloma híbrido (NK2) que produce un anticuerpo monoclonal que neutraliza el interferón leucocitario humano. Este anticuerpo permite una purificación de 5.000 veces de interferón mediante inmunoadsorción.

Área de la Ciencia:

Inmunología Inmunología.
La bioquímica es la bioquímica.

Sus antecedentes:

El interferón leucocitario humano posee propiedades antivirales.
Los anticuerpos monoclonales ofrecen una alta especificidad para las moléculas diana.
La purificación de los interferones es crucial para aplicaciones terapéuticas y de investigación.

Objetivo del estudio:

Para aislar un clon de mieloma híbrido secretando un anticuerpo monoclonal contra el interferón leucocitario humano.
Para caracterizar la capacidad del anticuerpo para neutralizar la actividad antiviral del interferón.
Desarrollar un inmunoadsorbente para una purificación eficiente del interferón.

Principales métodos:

La tecnología del hibridoma se utilizó para generar anticuerpos monoclonales.
La capacidad neutralizadora del anticuerpo se evaluó por su efecto sobre la actividad antiviral.
El anticuerpo estaba covalentemente unido a un soporte sólido para la inmunoadsorción.

Principales resultados:

Se aisló con éxito un clon de mieloma híbrido, designado NK2.
El anticuerpo monoclonal resultante neutralizó efectivamente la actividad antiviral del leucocito humano interferón.
Un proceso de inmunoadsorción en un solo paso utilizando el anticuerpo logró una purificación de interferón de hasta 5.000 veces.

Conclusiones:

El clon del hibridoma NK2 produce un potente anticuerpo monoclonal neutralizante contra el interferón leucocitario humano.
Este inmunoadsorbente basado en anticuerpos es una herramienta altamente efectiva para la purificación del interferón.

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