Video Experimental Relacionado
Updated: Jul 29, 2026
07:48
Investigating the Function of Coronin A in the Early Starvation Response of Dictyostelium discoideum by Aggregation Assays
Published on: June 18, 2016
Un transposón inusual que codifica las transcripciones inducibles al choque térmico y reguladas en el desarrollo en
Cell
|November 1, 1983
Resumen
Los investigadores identificaron un elemento genético móvil, Tdd-1, en el Dictyostelium discoideum. Este elemento transponible exhibe regulación del desarrollo y características estructurales únicas, ofreciendo información sobre la dinámica del genoma.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- Biología del desarrollo Biología del desarrollo.
Sus antecedentes:
- Los elementos transponibles (ET) son secuencias genéticas móviles que pueden alterar la estructura del genoma y la expresión génica.
- Dictyostelium discoideum sirve como organismo modelo para el estudio de los procesos celulares y de desarrollo de los eucariotas.
Objetivo del estudio:
- Para aislar y caracterizar un nuevo elemento transponible, Tdd-1, de Dictyostelium discoideum.
- Investigar las características estructurales, el número de copias y la distribución genómica de Tdd-1.
- Para analizar los patrones de expresión de Tdd-1 durante el desarrollo del Dictyostelium.
Principales métodos:
- Aislamiento y caracterización del elemento Tdd-1 utilizando técnicas moleculares.
- Análisis del Southern blot para evaluar la movilidad y el número de copias de Tdd-1 en diferentes cepas.
- Análisis de manchas del norte para estudiar la abundancia de transcripciones de Tdd-1 durante el desarrollo.
Principales resultados:
- Tdd-1 es un elemento transponible de 4,9 kb con 313 bp de repeticiones terminales invertidas, exhibiendo extensiones finales únicas.
- Aproximadamente 50 copias completas y 100-150 copias parciales de Tdd-1 están presentes por genoma haploide.
- Las manchas del sur confirmaron Tdd-1 como un elemento móvil dentro de Dictyostelium discoideum.
- Tdd-1 produce transcripciones reguladas por el desarrollo, que aumentan después de 10 horas de desarrollo, y una transcripción inducible por choque térmico de polaridad opuesta.
Conclusiones:
- Tdd-1 es un nuevo elemento transponible móvil en Dictyostelium discoideum con características estructurales distintas.
- La expresión del elemento está estrictamente regulada durante el desarrollo y responde al choque térmico.
- Tdd-1 representa una herramienta valiosa para el estudio de la dinámica del genoma y la regulación génica en Dictyostelium.
Videos de Conceptos Relacionados
02:57DNA-only Transposons
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
03:08LTR Retrotransposons
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
01:58Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
02:32Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
01:58Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
01:24Transposons
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...