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Conformación del ADN promotor: mapeo fino de los sitios hipersensibles a S1

E Schon, T Evans, J Welsh

|December 1, 1983

Resumen

Los investigadores mapearon los sitios hipersensibles a la S1-nucleasa en el ADN utilizando un método in vitro. Estos sitios, que se encuentran en regiones de genes activos, aparecen en el ADN superenrolado y se encuentran en secuencias específicas de ADN.

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética La genética.
La bioquímica es la bioquímica.

Sus antecedentes:

La cromatina activa presenta sitios hipersensibles a la S1-nucleasa en las regiones flanqueantes del gen 5', como lo muestran Larsen y Weintraub.
Estos sitios hipersensibles también se observan en plásmidos superenrollados que contienen secuencias de genes, pero no en los relajados.

Objetivo del estudio:

Desarrollar un método in vitro para mapear con precisión los sitios hipersensibles a la S1-nucleasa a nivel de nucleótidos.
Para investigar las características de estos sitios en el ADN desnudo.

Principales métodos:

Desarrollo de un enfoque in vitro utilizando círculos de ADN superenrollados generados artificialmente.
Mapeo del nivel de nucleótidos de los sitios S1-hipersensibles.

Principales resultados:

Los resultados del mapeo in vitro para el gen de beta-globina del pollo adulto coincidieron estrechamente con los datos del mapeo in vivo.
Los sitios hipersensibles identificados se encuentran dentro de los tractos homopurina-homopirimidina.
A pesar de la sensibilidad a la nucleasa S1, estos sitios no parecen ser de una sola hebra.

Conclusiones:

El método in vitro desarrollado mapea con precisión los sitios S1-hipersensibles.
Estos sitios en las regiones reguladoras de genes están asociados con el superenrolamiento del ADN y los motivos de secuencia específicos.
La naturaleza estructural de estos sitios requiere una mayor investigación.

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