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Múltiples ARNm de ubiquitina durante el desarrollo de Xenopus laevis contienen repeticiones en tándem de las 76

E Dworkin-Rastl, A Shrutkowski, M B Dworkin

|December 1, 1984

Resumen

Los investigadores identificaron un gen en Xenopus laevis que codifica para la ubiquitina, una proteína vital. Este gen existe en múltiples copias, lo que explica las variaciones en el tamaño del ARN mensajero durante el desarrollo.

Área de la Ciencia:

Biología del desarrollo Biología del desarrollo.
Biología Molecular Biología Molecular
Genética La genética.

Sus antecedentes:

Xenopus laevis es un organismo modelo para el estudio del desarrollo temprano.
La ubiquitina es una proteína altamente conservada involucrada en varios procesos celulares.
Los patrones de expresión génica cambian significativamente durante el desarrollo embrionario.

Objetivo del estudio:

Para caracterizar un clon de ADNc, pXlgC20, aislado de Xenopus laevis gastrulae. para caracterizar un clon de ADNc, pXlgC20, aislado de Xenopus laevis gastrulae. para caracterizar un clon de ADNc, pXlgC20, aislado de Xenopus laevis gastrulae.
Investigar la estructura y la función potencial del gen representado por pXlgC20.20.
Para comprender la base molecular de múltiples especies de ARN detectadas por el clon.

Principales métodos:

La construcción de la biblioteca de ADNc a partir del ARN poli (A) + del estadio 10 de Xenopus laevis gastrulae.
Análisis de hibridación para determinar las especies de ARN reconocidas por el clon.
Análisis de secuencia del clon de ADNc pXlgC20 para identificar unidades de repetición y marcos de codificación.
Comparación de la secuencia de aminoácidos deducida con proteínas conocidas.

Principales resultados:

El clon pXlgC20 contiene repeticiones en tándem de una secuencia de 228 bp.
Un marco de lectura potencial codifica para 76 unidades de aminoácidos en tándem.
La secuencia de aminoácidos deducida es idéntica a la ubiquitina humana.
El gen parece estar presente en múltiples copias en tándem en el genoma.
Múltiples clases de tamaño de ARNm (1600-3500 nucleótidos) se hibridan con el clon.

Conclusiones:

El clon pXlgC20 representa un gen que codifica la ubiquitina en el Xenopus laevis.
La ubiquitina en Xenopus laevis probablemente se tradujo de un precursor multimérico, similar a otras especies.
La variación observada en los tamaños de ARNm se atribuye a diferentes números de repeticiones de codificación de la ubiquitina.
Este hallazgo proporciona información sobre la organización génica y la regulación de la expresión durante el desarrollo de Xenopus.

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