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Splicing de ARN precursor ribosómico grande y el procesamiento de ARN intrón en las mitocondrias de levadura
Cell
|December 1, 1984
Resumen
El empalme de ARN mitocondrial de levadura involucra intermediarios como ARN lineales y circulares. El intrón extirpado puede circular o someterse a una escisión interna, similar a la autoescisión de Tetrahymena.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La expresión génica mitocondrial en la levadura implica un complejo procesamiento de ARN.
- La síntesis y la maduración del ARN ribosomal (ARNr) son críticas para la función celular.
Objetivo del estudio:
- Para investigar el mecanismo de empalme de los mitocondriales de levadura grandes precursores de ARN ribosomal.
- Para caracterizar las vías de procesamiento de los intrones extirpados.
Principales métodos:
- Extensión de primer con transcriptasa inversa.
- Microscopía electrónica para visualizar las estructuras de ARN.
Principales resultados:
- Intermedios de ARN lineal identificados con una G. 5'-terminal no codificada.
- Intermedios circulares de ARN descritos formados por enlaces covalentes de las fronteras de intrones.
- Se observó que tres nucleótidos de la frontera del intrón 5' están ausentes en la forma circularizada.
- Demostró que los intrones extirpados pueden circular o sufrir una escisión interna.
Conclusiones:
- Los intermediarios de empalme observados y las vías de procesamiento son consistentes con un mecanismo de autoemplazamiento, similar al ARNr nuclear de Tetrahymena.
- Los intrones mitocondriales de la levadura exhiben un doble destino de procesamiento: circularización o escisión interna.
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