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El crecimiento celular con ácidos grasos trans se ve afectado por el adenosina 3',5'-monofosfato y la fluidez de la
Science (New York, N.Y.)
|February 15, 1980
Resumen
Los ácidos grasos trans apoyan el crecimiento de Escherichia coli cuando se complementan con adenosina 3 3 .
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Microbiología Microbiología.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Los mutantes de Escherichia coli requieren ácidos grasos insaturados para el crecimiento.
- Los isómeros de ácidos grasos (cis y trans) influyen en la fluidez de la membrana y en los procesos celulares.
- El adenosina 3',5'-monofosfato (cAMP) es un segundo mensajero crucial que regula las vías metabólicas.
Objetivo del estudio:
- Para investigar la respuesta de crecimiento de un mutante de E. coli a los ácidos grasos insaturados trans vs. cis.
- Para determinar el efecto de la suplementación de adenosina 3',5'-monofosfato en el rendimiento de crecimiento con diferentes isómeros de ácidos grasos.
- Aclarar el mecanismo por el cual los ácidos grasos afectan la regulación celular.
Principales métodos:
- Cultivo de una E. coli mutante auxotrófica de ácidos grasos insaturados en un medio de glucosa.
- Medios complementarios con isómeros específicos de octadecenoato trans o cis.
- Concentraciones variadas de adenosina 3',5'-monofosfato.
- Medir el rendimiento celular y la incorporación de ácidos grasos en fosfolípidos de membrana.
Principales resultados:
- Los trans octadecenoatos no apoyaron el crecimiento, pero aumentaron significativamente el rendimiento celular con la suplementación con adenosina 3',5'-monofosfato.
- Los isómeros cis apoyaban altos rendimientos celulares independientemente de la adenosina 3',5'-monofosfato.
- La incorporación de isómeros trans en fosfolípidos fue rápida, pero no explicó completamente el crecimiento mejorado con cAMP.
- Las células cultivadas con ácidos grasos trans exhibieron un mayor requerimiento de adenosina 3',5'-monofosfato.
Conclusiones:
- La suplementación con adenosina 3',5'-monofosfato puede rescatar los defectos de crecimiento causados por los ácidos grasos trans en E. coli.
- Los ácidos grasos trans pueden alterar la regulación metabólica normalmente controlada por la adenosina 3',5'-monofosfato.
- Esto sugiere una compleja interacción entre la estructura de los ácidos grasos, las propiedades de la membrana y la señalización de nucleótidos cíclicos.
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