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El virus de la leucemia por radiación contiene dos ARN virales distintos
Cell
|March 1, 1980
Resumen
Los investigadores analizaron el genoma de ARN de RadLV/VL3, un virus oncogénico de la leucemia murina. Identificaron distintos componentes de ARN y encontraron que la subunidad de 5.6 kb carece del gen p30, lo que explica sus propiedades oncogénicas.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Oncología Oncología.
Sus antecedentes:
- RadLV/VL3 es un virus de la leucemia murina altamente oncogénico.
- Se origina en un linfoma timo inducido por el virus de la leucemia por radiación en ratones C57BL/Ka.
- Un retrovirus nononcogénico, BL/Ka(B), es endógeno en la misma cepa de ratón.
Objetivo del estudio:
- Para analizar el genoma de ARN de RadLV/VL3.3.
- Para comparar sus componentes de ARN con un retrovirus nononcogénico.
- Comprender las bases genéticas de la oncogenicidad de RadLV/VL3.
Principales métodos:
- Análisis del genoma de ARN de los viriones RadLV/VL3.
- Identificación y caracterización de los componentes del ARN viral (dimeros 70S y 54S).
- Análisis de la plantilla de primeras de transcriptasa inversa y síntesis de ADNc.
- Traducción in vitro de las subunidades de ARN y análisis de proteínas.
- Estudios de hibridación de ARN y ARN.
Principales resultados:
- Los viriones RadLV/VL3 contienen 70S (8 subunidades de kb) y 54S (5,6 subunidades de kb) dimeros de ARN.
- BL/Ka(B) contiene sólo 8 kb de subunidades de ARN viral.
- La subunidad de 5,6 kb de RadLV/VL3 carece del gen para la proteína p30 pero contiene genes para p15 y p12.
- La traducción in vitro del ARN de 5,6 kb produjo una proteína que reaccionó con anticuerpos anti-p15 pero no anti-p30.
- Se detectó un nuevo ARN citoplasmático que contiene 1,6 kb de poli (A) en células productoras de RadLV/VL3 con baja homología a BL/Ka (B).
Conclusiones:
- La deleción o ausencia del gen p30 en la subunidad de ARN de 5,6 kb probablemente contribuye a la alta oncogenicidad de RadLV/VL3.
- La distinta composición de ARN y el nuevo ARN citoplasmático sugieren mecanismos únicos en la patogénesis de RadLV/VL3.
- El análisis genómico comparativo proporciona información sobre la oncogénesis retroviral.
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