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La proteína transformadora del virus del sarcoma aviar, pp60src muestra actividad de proteína quinasa específica para
Nature
|May 15, 1980
Resumen
El phosphoprotein pp60src del virus del sarcoma aviar (VSA) es una proteína quinasa. Esta proteína quinasa fosforila los residuos de tirosina en varios sustratos, jugando un papel clave en la transformación maligna.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Virología Virología.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Los virus del sarcoma aviar (VSA) causan una transformación maligna.
- La proteína ASV pp60src está implicada en esta transformación.
- Anteriormente se sugirió el papel de pp60src en la fosforilación de proteínas celulares.
Objetivo del estudio:
- Para purificar y caracterizar bioquímicamente la proteína transformadora ASV pp60src.
- Para determinar si pp60src posee actividad enzimática.
- Para identificar el tipo específico de fosforilación mediada por pp60src.
Principales métodos:
- Purificación bioquímica de pp60src utilizando el intercambio iónico y la cromatografía de inmunoafinidad.
- Análisis de la actividad de la proteína quinasa en preparados pp60src purificados.
- Análisis de los residuos fosforilados en las proteínas del sustrato.
Principales resultados:
- pp60src fue purificado aproximadamente 5.000 veces.
- La pp60src purificada exhibe actividad cíclica de la proteína quinasa independiente de la AMP.
- Esta actividad de la quinasa fosforila específicamente los residuos de tirosina en varios sustratos de proteínas.
Conclusiones:
- El producto del gen transformador de ASV, pp60src, es una proteína quinasa.
- pp60src media en la fosforilación de los residuos de tirosina.
- Esta actividad de la tirosina quinasa es probablemente central para la transformación maligna inducida por el ASV.
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