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El ARN mensajero se traduce cuando está asociado con el marco citoesquelético en células HeLa normales e infectadas

M Cervera, G Dreyfuss, S Penman

|January 1, 1981

Resumen

La célula La célula es la célula.

Área de la Ciencia:

Biología celular Biología celular.
Biología Molecular Biología Molecular
La bioquímica es la bioquímica.

Sus antecedentes:

El citoesqueleto juega un papel crucial en la organización y función celular.
La traducción del ARN mensajero (ARNm) es un proceso fundamental para la síntesis de proteínas.

Objetivo del estudio:

Investigar la asociación de los poliribosomas y el ARNm con el marco citoesquelético.
Comprender el papel del citoesqueleto en la localización y traducción del ARNm, incluso durante la infección viral.

Principales métodos:

Las células HeLa se extrajeron con detergente no iónico para aislar el marco citoesquelético.
Se fraccionaron los poliribosomas, el tRNA, los monoribosomas y las proteínas.
El etiquetado de búsqueda de pulsos se utilizó para rastrear la dinámica del ARNm viral en células infectadas por VSV.

Principales resultados:

Los poliribosomas y el ARNm de traducción activa están predominantemente unidos al marco citoesquelético.
El ARNm viral se asocia inicialmente con el citoesqueleto durante la infección por VSV, pero luego se desprende.
El ARNm asociado al retículo endoplasmático, incluida la glicoproteína G viral, es retenido por la estructura extraída del detergente.

Conclusiones:

La asociación citoesquelética puede ser esencial para la traducción del ARNm.
La subestructura proteica del retículo endoplasmático involucrada en la unión de los poliribosomas es parte del marco citosquelético.
La dinámica del ARNm viral, incluida la traducción y la localización, está influenciada por el marco citoesquelético de la célula huésped.

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