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Las proteínas transformadoras de algunos virus del sarcoma felino y aviar están relacionadas estructural y

|April 1, 1981

Resumen

Los virus del sarcoma aviar (VSA), como el virus del sarcoma de Fujinami (FSV), elevan los niveles celulares de fosfotirosina, lo que indica un papel en la transformación celular. Estos virus poseen actividad de proteína quinasa específica para la tirosina, lo que sugiere distintas clases virales involucradas en la fosforilación de proteínas aberrantes.

Área de la Ciencia:

* Biología Molecular.
* Virología Virología.
* Oncología Oncología.

Sus antecedentes:

* Se sabe que los virus del sarcoma aviar (VSA) causan tumores en pollos.
* Algunos ASVs, como el virus del sarcoma de Rous (RSV), poseen un gen src que codifica una proteína quinasa específica de tirosina.
* Las proteínas transformadoras de ciertos ASV están asociadas con la actividad de la proteína quinasa específica de la tirosina.

Objetivo del estudio:

* Para investigar el papel de los niveles de fosfotirosina en la transformación celular inducida por el virus del sarcoma de Fujinami (FSV), PRC II y Y73.
* Para caracterizar la actividad de la proteína quinasa de las proteínas relacionadas con la mordaza codificadas por estos ASVs.
* Para comparar las proteínas transformadoras de FSV, PRC II y Y73 con las de otros retrovirus oncogénicos conocidos.

Principales métodos:

* Ensayos de transformación celular utilizando FSV, PRC II y Y73.
* Medición de los niveles de fosfotirosina en las proteínas celulares.
* Ensayos de quinasa del complejo inmune para determinar la actividad de la proteína quinasa.
* Cartografía de péptidos trípticos y estudios inmunológicos para la comparación estructural de proteínas.

Principales resultados:

* FSV, PRC II y Y73 indujeron un aumento de 4 a 13 veces en los niveles celulares de fosfotirosina.
* Las proteínas relacionadas con la mordaza de estos ASVs exhibieron actividad de proteína quinasa específica de tirosina y fueron fosforiladas in vivo.
* Las proteínas transformadoras FSV y PRC II mostraron similitud estructural con las proteínas del virus del sarcoma felino (FeSV), pero no con las proteínas RSV o Y73.
* La actividad de la proteína quinasa del FSV y los niveles de fosfotirosina fueron sensibles a la temperatura.

Conclusiones:

* La fosforilación aberrante de las proteínas celulares en los residuos de tirosina es un mecanismo común en la transformación celular inducida por ASV.
* Al menos cuatro clases distintas de retrovirus pueden utilizar la fosforilación de tirosina para su transformación.
* FSV, PRC II y FeSV representan una clase, mientras que RSV, Y73 y el virus de la leucemia murina de Abelson representan otras clases distintas.

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