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Un control de efecto de posición para la transposición génica: el estado de expresión de los genes de tipo de
Cell
|August 1, 1981
Resumen
La conmutación del tipo de apareamiento de la levadura normalmente implica una transferencia génica unidireccional al locus MAT. Sin embargo, cuando se expresan los loci del tipo de apareamiento silencioso (HML y HMR), cambian de forma eficiente, independientemente de su ubicación.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- Biología de la levadura Biología de la levadura.
Sus antecedentes:
- El cambio de tipo de apareamiento en Saccharomyces cerevisiae es un reordenamiento programado del ADN.
- Este proceso generalmente implica la transposición unidireccional de genes de tipo de apareamiento de los loci silenciosos (HML, HMR) al locus MAT expresado.
- Los loci silenciosos HML y HMR están generalmente protegidos de la conmutación.
Objetivo del estudio:
- Para investigar el papel de la expresión del locus en la direccionalidad de la conmutación del tipo de apareamiento.
- Para determinar si los loci silenciosos expresados cambian a velocidades comparables a las del locus MAT.
- Para entender el control de efecto de posición sobre la direccionalidad de transposición.
Principales métodos:
- Análisis de las tasas de cambio de tipo de apareamiento en cepas de levadura de tipo silvestre y mutantes (por ejemplo, mar mutantes).
- Monitoreo de eventos de transposición en las localizaciones de MAT, HML y HMR.
- Evaluación del estado de expresión de los loci de tipo de apareamiento.
Principales resultados:
- En la levadura de tipo silvestre, el cambio es unidireccional, con sólo el locus MAT siendo reemplazado.
- En las cepas donde se expresan los loci HML y HMR (por ejemplo, los mutantes mar), estos loci también experimentan una conmutación eficiente.
- Las tasas de conmutación para los loci HML y HMR expresados son equivalentes a las del locus MAT.
Conclusiones:
- El estado de expresión de un locus de tipo de apareamiento es un determinante crítico de su susceptibilidad al cambio.
- El control de efecto de posición sobre la direccionalidad de transposición está modulado por la expresión de locus.
- Los loci de tipo de apareamiento expresados se convierten eficientemente independientemente de su ubicación genómica.
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