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Estructura y evolución de los genes de gamma, beta C y beta A-globina de cabra: tres genes regulados en el desarrollo
Cell
|December 1, 1981
Resumen
La secuenciación genética de globina de cabra revela una alta homología entre las formas fetal, preadulta y adulta. Las inserciones únicas en los intrones sugieren divergencias recientes y elementos similares a los transposones, con el gen gamma teniendo un papel específico para el feto.
Área de la Ciencia:
- La genómica es la genómica.
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- Los genes de la globina son cruciales para el transporte de oxígeno y exhiben una expresión específica de la etapa del desarrollo.
- Comprender la evolución de los genes de las globinas proporciona información sobre la regulación genética y la especiación.
Objetivo del estudio:
- Determinar las secuencias completas de nucleótidos de los genes de globina fetal (gamma), preadulta (beta C) y adulta (beta A) de cabra.
- Para analizar las relaciones evolutivas y las diferencias estructurales entre estos genes de globina de cabra.
Principales métodos:
- Secuenciación completa de nucleótidos de los genes de globina gamma, beta C y beta A de cabra.
- Análisis comparativo de secuencias con pseudogenes de globina de cabra (psi beta X, psi beta Z).
Principales resultados:
- Los genes de globina fetal, preadulto y adulto de cabra muestran una alta homología (~ 90%) en las regiones codificantes y no codificantes.
- Las principales diferencias se atribuyen a las inserciones en intrones grandes: gamma (247 bp), beta A (318 bp) y beta C (252 bp de inserción, 60 bp de deleción).
- El gen gamma de la cabra funciona específicamente en la etapa fetal, a diferencia de los genes de globina fetal humana.
Conclusiones:
- Los genes de las globinas gamma, beta A y beta C de cabra han divergido recientemente.
- Los eventos de duplicación genética que involucran pares de genes ancestrales pseudo-funcionales probablemente formaron psi beta X-beta C y psi beta Z-beta A.
- Las inserciones intrónicas exhiben características similares a las de los transposones y están muy extendidas en el genoma de la cabra.
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