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Organización molecular de un sitio de hinchazón de Drosophila que responde a la ecdisona
Cell
|January 1, 1982
Resumen
El locus 68C en las glándulas salivales de Drosophila melanogaster contiene genes para proteínas de pegamento, incluyendo sgs3. Estos genes se transcriben durante el desarrollo larval y están regulados por la ecdisona, con un elemento transponible que no muestra ningún efecto en la expresión génica.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética molecular La genética molecular.
- Drosophila melanogaster Investigación La búsqueda La búsqueda La búsqueda La búsqueda La búsqueda La búsqueda
Sus antecedentes:
- El locus 68C en las glándulas salivales de Drosophila melanogaster es una bocanada transcripcionalmente activa durante las últimas etapas larvales.
- Esta hinchazón está asociada con la síntesis de polipéptidos de pegamento, incluido sgs3, esencial para la puparización.
- La actividad de la hinchazón está regulada por la hormona esteroide ecdisona, que induce la regresión.
Objetivo del estudio:
- Para caracterizar molecularmente el locus de la hinchazón 68C en Drosophila melanogaster.
- Identificar y mapear los genes responsables de la síntesis de polipeptídios de la cola de las glándulas salivales.
- Para investigar el papel de los elementos transponibles en la regulación génica en este locus.
Principales métodos:
- Aislamiento y caracterización de segmentos de ADN superpuestos que definen el locus de inhalación 68C.
- Identificación y mapeo de ARN transcritos utilizando el Northern blotting y la hibridación.
- Análisis de la organización, orientación y potenciales elementos reguladores de los genes.
Principales resultados:
- Aproximadamente 50 kb de ADN en el locus 68C fueron clonados y analizados.
- Se identificaron tres ARN poli (A) + principales (genes II, III y IV) dentro de un grupo de 5 kb, específico de las glándulas salivales durante la etapa de hinchazón.
- El gen IV (1.1 kb ARN) fue identificado como el gen estructural sgs3; los genes II y III forman un par potencialmente co-regulado.
- Una inserción de elemento transponible (roo) adyacente al grupo de genes no afectó la transcripción de los genes II, III y IV.
Conclusiones:
- El locus 68C contiene un conjunto estrechamente agrupado de genes involucrados en la función de las glándulas salivales, incluyendo sgs3.
- Los genes II y III pueden representar una unidad transcripcional corregida con elementos reguladores potenciales.
- La inserción de elementos transponibles en este locus no parece influir en la expresión de genes clave del desarrollo en las glándulas salivales.
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