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Secuencias múltiples relacionadas con un gen de cadena de luz kappa de región constante en el genoma del conejo

O Heidmann, F Rougeon

|March 1, 1982

Resumen

Los alotipos de cadena ligera del conejo kappa (b4, b5, b6, b9) están controlados por múltiples genes. El análisis del ADN revela una organización genética distinta para cada alotipo, apoyando una hipótesis de duplicación de genes polimórficos para su control.

Área de la Ciencia:

Inmunogenética La inmunogenética.
Biología Molecular Biología Molecular
La genómica comparada es una genómica comparada.

Sus antecedentes:

Cuatro genes alélicos controlan los alotipos de la cadena ligera kappa del conejo (b4, b5, b6, b9).
Existe una divergencia significativa de aminoácidos en la región de la constante de kappa.
Estudios anteriores indicaron la posibilidad de que los conejos expresen alotipos incorrectos.

Objetivo del estudio:

Para investigar la hipótesis de que los alotipos b del conejo están codificados por genes duplicados.
Para explorar un mecanismo de control polimórfico para estos genes duplicados.
Para analizar la organización del ADN de diferentes alotipos de la cadena ligera kappa.

Principales métodos:

Hibridación de la mancha del sur utilizando una sonda kappa b4 C clonada.
La digestión del ADN con las endonucleasas de restricción (Eco RI, Kpn I, Pst I).
Análisis del ADN de cuatro tipos de conejos homocigotos (b4, b5, b6, b9).

Principales resultados:

Todos los ADN de conejo analizados contenían múltiples fragmentos de ADN que se hibridaron con la sonda C kappa.
Se observaron patrones de restricción distintos para cada alotipo.
La hibridación se produjo tanto en condiciones de lavado de baja como de alta rigidez.

Conclusiones:

Los genes de la región constante de kappa (C kappa) se organizan de manera diferente en conejos que expresan diferentes alotipos.
Los hallazgos apoyan la hipótesis de genes duplicados con control polimórfico para los alotipos b.
Sugiere una base genética compleja para la determinación del alotipo de la cadena ligera kappa en conejos.

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