Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

17.3K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
17.3K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

9.1K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.1K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

14.6K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
14.6K
Bacterial Transcription01:53

Bacterial Transcription

25.6K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
25.6K
Transcription Initiation01:47

Transcription Initiation

17.0K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
17.0K
Transcription in Prokaryotes01:28

Transcription in Prokaryotes

4.4K
Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow...
4.4K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

A new oil/membrane approach for integrated sweat sampling and sensing: sample volumes reduced from μL's to nL's and reduction of analyte contamination from skin.

Lab on a chip·2016
Same author

Extracellular matrix microarrays to study inductive signaling for endoderm specification.

Acta biomaterialia·2016
Same author

The microfluidics of the eccrine sweat gland, including biomarker partitioning, transport, and biosensing implications.

Biomicrofluidics·2015
Same author

Nucleotide modification of tRNA in. the yeast Saccharomyces cerevisiae is not Affected by the ψ factor which modulates suppression efficiency.

Current genetics·2013
Same author

Nuclear mobility and mitotic chromosome binding: similarities between pioneer transcription factor FoxA and linker histone H1.

Cold Spring Harbor symposia on quantitative biology·2011
Same author

Climate change and the integrity of science.

Science (New York, N.Y.)·2010

Video Experimental Relacionado

Updated: May 5, 2026

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
12:12

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Published on: March 12, 2017

9.3K

Secuencia de ADN requerida para la terminación eficiente de la transcripción en levaduras.

K S Zaret, F Sherman

    Cell
    |March 1, 1982
    PubMed
    Resumen

    Una mutación de la levadura (cyc1-512) interrumpe la terminación de la transcripción, lo que lleva a niveles alterados del producto del gen CYC1 y al ARNm. Esto sugiere que la poliadenilación está acoplada a la terminación de la transcripción en la levadura.

    Más Videos Relacionados

    DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
    09:12

    DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

    Published on: September 16, 2019

    8.2K
    Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
    09:15

    Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

    Published on: May 9, 2020

    6.1K

    Videos de Experimentos Relacionados

    Last Updated: May 5, 2026

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
    12:12

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

    Published on: March 12, 2017

    9.3K
    DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
    09:12

    DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

    Published on: September 16, 2019

    8.2K
    Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
    09:15

    Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

    Published on: May 9, 2020

    6.1K

    Área de la Ciencia:

    • Biología Molecular Biología Molecular
    • Genética La genética.
    • Biología de la levadura Biología de la levadura.

    Sus antecedentes:

    • El gen CYC1 en Saccharomyces cerevisiae codifica el iso-1-citocromo c.
    • La terminación adecuada de la transcripción es crucial para la regulación génica y la prevención de la inestabilidad genómica.

    Objetivo del estudio:

    • Investigar las consecuencias funcionales de la mutación cyc1-512.
    • Determinar el papel de la región 3' no traducida en la expresión génica CYC1 y la terminación de la transcripción.

    Principales métodos:

    • Caracterización del mutante de deleción cyc1-512 en la levadura.
    • Análisis de los niveles de ARNm CYC1, el tamaño y la poliadenilación.
    • Investigación de los patrones de transcripción en el locus CYC1.

    Principales resultados:

    • La mutación cyc1-512, una deleción de 38 bp, reduce los niveles de iso-1-citocromo c y CYC1 mRNA.
    • Los extremos 3' anormalmente largos del ARNm CYC1 indican una terminación fallida de la transcripción.
    • Se observó una transcripción convergente entre CYC1 y un gen adyacente.

    Conclusiones:

    • La deleción de cyc1-512 perjudica la terminación de la transcripción de la levadura.
    • La poliadenilación puede ser acoplada a la terminación de la transcripción en la levadura.
    • Una secuencia conservada en la región no traducida de 3' podría estar involucrada en la terminación.