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Videos de Conceptos Relacionados

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

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Nucleotide Excision Repair01:08

Nucleotide Excision Repair

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Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Video Experimental Relacionado

Updated: Jul 18, 2026

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

La endonucleasa T4 VII divide las estructuras holliday.

K Mizuuchi, B Kemper, J Hays

    Cell
    |June 1, 1982
    PubMed
    Resumen

    La endonucleasa T4 VII rompe las estructuras de Holliday, resolviendo los intermediarios de la replicación del ADN. Su acción con la ligasa de ADN produce productos genéticamente sensibles, lo que aclara su papel en el metabolismo del ADN de los fagos T4.

    Área de la Ciencia:

    • Biología Molecular Biología Molecular
    • Enzimología Enzimología.
    • Replicación y reparación del ADN.

    Sus antecedentes:

    • Las estructuras de Holliday son intermediarios clave en la recombinación y reparación del ADN.
    • La T4 endonucleasa VII está involucrada en el metabolismo del ADN del bacteriófago T4.

    Objetivo del estudio:

    • Para aclarar el mecanismo por el cual la T4 endonucleasa VII resuelve las estructuras de Holliday.
    • Comprender el papel de la T4 endonucleasa VII en la generación de productos de ADN genéticamente sensibles.

    Principales métodos:

    • Ensayos de escisión in vitro utilizando endonucleasa VII T4 purificada.
    • Análisis de productos de ADN generados por la actividad de la endonucleasa VII.

    Principales resultados:

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    Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
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    • La endonucleasa T4 VII específicamente divide ambas hebras de las estructuras de Holliday en o cerca del punto de ramificación.
    • La escisión genera dos duplexos de ADN no ramificados, cada uno con una ruptura de una sola hebra.
    • Las rupturas de cadena ocurren en posiciones idénticas dentro de la secuencia de nucleótidos en cada cadena.

    Conclusiones:

    • La T4 endonucleasa VII actúa en el punto de ramificación del ADN dúplex, resolviendo las estructuras de Holliday.
    • La acción combinada de la T4 endonucleasa VII y la ligasa de ADN puede reparar las rupturas de las hebras, produciendo ADN funcional.
    • Estos hallazgos explican la función de la enzima en el metabolismo del ADN del fago T4 e identifican como la primera enzima con actividad específica del punto de ramificación.