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Las proteínas transformadoras del virus del sarcoma de Rous, el virus del sarcoma de Harvey y el virus de Abelson
Cell
|December 1, 1982
Resumen
Las proteínas transformadoras del virus del sarcoma de Rous, el virus del sarcoma de Harvey y el virus de Abelson se unen a los lípidos. Esta modificación lipídica, particularmente en p60src y p21, puede permitir la asociación de membrana para estas proteínas virales.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Virología Virología.
- Biología celular Biología celular.
Sus antecedentes:
- Los virus oncogénicos utilizan proteínas transformadoras para inducir cambios celulares.
- El mecanismo por el cual las proteínas de transformación viral se asocian con las membranas celulares no se entiende completamente.
Objetivo del estudio:
- Para investigar el papel de la modificación lipídica en la unión a la membrana de las proteínas transformadoras virales.
- Para determinar la ubicación y la importancia de la fijación lipídica a proteínas virales específicas.
Principales métodos:
- Análisis de las proteínas transformadoras del virus del sarcoma de Rous (p60src), el virus del sarcoma de Harvey (p21) y el virus de Abelson.
- Análisis bioquímicos para detectar y localizar lípidos fuertemente unidos.
Principales resultados:
- Se encontró que las proteínas transformadoras de los tres virus (sarcoma de Rous, sarcoma de Harvey, Abelson) contenían lípidos fuertemente unidos.
- La modificación lipídica se observó en las formas maduras de p60src y p21, pero no en la p21 recientemente sintetizada.
- El lípido asociado con p60src se encuentra en el dominio N-terminal, implicado en la unión a la membrana.
Conclusiones:
- La adición de lípidos posttranslacional es un mecanismo probable para que las proteínas virales transformadoras adquieran afinidad de membrana.
- La modificación lipídica es crucial para la asociación de membrana de las proteínas p60src y p21 maduras.
- Esta modificación lipídica puede ser esencial para la función oncogénica de estas proteínas virales.
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