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¿Es la ribonucleotide reductasa la función transformadora del virus del herpes simple 2?
Nature
|March 3, 1983
Resumen
La transformación del virus del herpes simple 2 (HSV-2) puede involucrar a la ribonucleótido reductasa. Un anticuerpo monoclonal dirigido a proteínas potencialmente transformadoras también identificó esta enzima, lo que sugiere su papel en la transformación celular inducida por HSV-2.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Investigación del cáncer Investigación del cáncer.
Sus antecedentes:
- El virus del herpes simple 2 (HSV-2) puede inducir la transformación celular.
- Los fragmentos específicos del genoma viral (BglII C y N) están implicados en este proceso.
- Estos fragmentos codifican polipéptidos potencialmente involucrados en la transformación.
Objetivo del estudio:
- Para investigar el papel de la ribonucleotide reductasa inducida por HSV-2 en la transformación viral.
- Para identificar las proteínas virales específicas asociadas con la transformación.
Principales métodos:
- Purificación parcial de la ribonucleótido reductasa inducida por el HSV-2.
- Criar un antisuero de conejo contra la enzima purificada.
- El uso de un anticuerpo monoclonal contra las putativas proteínas transformadoras.
- Pruebas de inmunoprecipitación para identificar las interacciones proteína-antígeno.
Principales resultados:
- El antisuero de conejo inhibió la actividad de la reductasa e inmunoprecipitó los polipéptidos 144K y 38K.
- Un anticuerpo monoclonal compitió con el antisuero por estos antígenos.
- El anticuerpo monoclonal especificamente inmunoprecipitado redujo la actividad de la reductasa.
Conclusiones:
- Se sugiere fuertemente que la ribonucleotide reductasa juega un papel en la transformación celular inducida por el HSV-2.
- Los polipéptidos 144K y 38K identificados están probablemente relacionados con la ribonucleótido reductasa.
- Este hallazgo proporciona un mecanismo molecular potencial para la oncogénesis mediada por HSV-2.
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