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Updated: May 6, 2026

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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
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Los oligosacáridos enlazados con O son adquiridos por las glicoproteínas del virus del herpes simple en el aparato de
Cell
|March 1, 1983
Resumen
Las glicoproteínas del virus del herpes simple tipo 1 (HSV1) se someten a una glicosilación vinculada a la O al final del aparato de Golgi. Esta modificación afecta significativamente su movilidad electroforética y es crucial para la maduración de la glicoproteína viral.
Área de la Ciencia:
- Virología Virología.
- Glucobiología Glucobiología.
- Biología celular Biología celular.
Sus antecedentes:
- Las glicoproteínas del virus del herpes simple tipo 1 (HSV1) sufren extensas modificaciones post-traduccionales.
- Comprender estas modificaciones es clave para comprender la patogénesis del HSV1 y desarrollar antivirales.
Objetivo del estudio:
- Para caracterizar los oligosacáridos vinculados a la O en las glicoproteínas HSV1 maduras.
- Para aclarar la relación temporal entre la glicosilación ligada a O y otras modificaciones post-traducionales.
Principales métodos:
- Análisis electroforético de las glicoproteínas del HSV1.
- Etiquetado metabólico y experimentos de búsqueda de pulsos.
- Estudios de inhibición utilizando monensina y una línea celular resistente a la ricina.
Principales resultados:
- Los oligosacáridos enlazados con O contribuyen significativamente a la menor movilidad electroforética de las glicoproteínas HSV1 maduras.
- La acilación de ácidos grasos precede a la adquisición de oligosacáridos enlazados con O.
- La conversión de oligosacáridos ligados a N en tipos complejos coincide con la glicosilación ligada a O.
Conclusiones:
- La extensión y la unión de oligosacáridos enlazados con O en las glicoproteínas del HSV1 ocurren como un evento postraslacional tardío.
- Este proceso tiene lugar en el aparato de Golgi.
- La glicosilación ligada a O depende de la maquinaria celular para el procesamiento de oligosacáridos ligados a N.
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