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Un elemento transponible insertado sólo 5' a un gen de proteína de pegamento de Drosophila altera la expresión génica

W McGinnis, A W Shermoen, S K Beckendorf

|August 1, 1983

Resumen

Un nuevo elemento transponible, el hobo, interrumpe la regulación génica de Drosophila Sgs-4. Esta interrupción reduce la producción de proteínas de pegamento y altera los patrones de expresión génica en las glándulas salivales.

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética La genética.
Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

El gen Drosophila Sgs-4 es crucial para la producción de proteínas de pegamento en larvas maduras.
La expresión génica de Sgs-4 está regulada por una remota región aguas arriba sensible a la digestión de la DNAasa I.

Objetivo del estudio:

Para investigar el impacto de una inserción de ADN en la expresión y regulación del gen Sgs-4.
Para caracterizar una nueva familia de elementos transponibles, denominados hobo.

Principales métodos:

Análisis de una variante del locus Sgs-4 que contiene una inserción de 1,3 kb de ADN.
Ensayos de hipersensibilidad de la DNAasa I para mapear regiones reguladoras.
Análisis de transcripción para identificar productos genéticos Sgs-4.

Principales resultados:

Se encontró una inserción de 1,3 kb de ADN, identificada como el elemento transponible hobo, entre el gen Sgs-4 y su región reguladora remota.
La expresión de la variante Sgs-4 locus se redujo 50-100 veces.
Se detectaron cuatro transcripciones del locus variante, con dos iniciaciones dentro del elemento hobo, a diferencia de la única transcripción normal.
La regulación del desarrollo se mantuvo específica para las glándulas salivales larvales tardías, a pesar de los sitios de iniciación de transcripción alterados.
Se formaron nuevos sitios hipersensibles de la DNAasa I dentro del elemento vagabundo, que se correlacionan con los nuevos sitios de inicio de transcripción.

Conclusiones:

El elemento transponible hobo puede interrumpir significativamente la expresión génica y alterar los patrones de transcripción.
A pesar de los cambios en la iniciación de transcripción, la regulación del desarrollo de Sgs-4 permanece intacta.
El estudio identifica nuevos mecanismos reguladores asociados con los elementos transponibles y la expresión génica.

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