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Linfomas T murinos en los que el oncogén mic celular ha sido activado por inserción retroviral
Cell
|May 1, 1984
Resumen
Las inserciones retrovirales activan el myc oncogene en los cánceres de linfocitos T. Esto probablemente ocurre a través de elementos potenciadores en la repetición terminal larga retroviral (LTR), lo que lleva a un aumento de la expresión micológica y el desarrollo de tumores.
Área de la Ciencia:
- Oncología Oncología Oncología.
- Virología Virología.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El myc oncogene juega un papel crítico en el desarrollo de cánceres de linfocitos T.
- Los virus de la leucemia murina y los retrovirus recombinantes (virus MCF) están implicados en la leucemogénesis.
- La activación de los oncogenes es un mecanismo clave en el desarrollo del cáncer.
Objetivo del estudio:
- Investigar el papel de las inserciones retrovirales en la activación del oncogén c-myc en la neoplasia de linfocitos T.
- Identificar el mecanismo por el cual c-myc se activa en los linfomas T inducidos por retrovirus.
Principales métodos:
- Clonado de inserciones retrovirales en relación con el gen c-myc en linfomas T.
- Análisis de los niveles de c-myc mRNA en muestras de tumores.
- Secuenciación de la repetición terminal larga retroviral (LTR) y los genes env.
Principales resultados:
- Se identificaron inserciones retrovirales de 5' a c-myc en linfomas T inducidos por el virus de la leucemia murina de Soule y en un linfoma espontáneo de ratón AKR.
- Los niveles de ARNm c-mico del tumor fueron aproximadamente 5 veces más altos en los linfomas con inserciones en comparación con aquellos sin.
- Se encontraron inserciones similares a MCF cerca de c-myc en una proporción significativa de los linfomas AKR T, lo que sugiere un mecanismo común.
Conclusiones:
- Las inserciones retrovirales, que probablemente actúan a través de potenciadores de LTR, activan el oncogén c-myc en una cuarta parte de los linfomas T inducidos por retrovirus.
- Esta activación conduce a una mayor expresión de c-myc y contribuye a la neoplasia de linfocitos T.
- Los hallazgos destacan la importancia de la actividad del potenciador de LTR en la activación de oncogenes y la leucemogénesis.
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