Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Chromosome Structure02:40

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

The 5' end of yeast 5.8S rRNA is generated by exonucleases from an upstream cleavage site.

The EMBO journal·1994
Same author

Recombination and RNA processing: a common strand?

Trends in cell biology·1991
Same author

The SFL2 (TUP1?) protein of Saccharomyces cerevisiae contains a repeating motif homologous to beta subunits of G proteins.

Gene·1991
Same author

Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeast.

Cell·1984
Same author

Analysis of sequences conferring autonomous replication in baker's yeast.

The EMBO journal·1983

Video Experimental Relacionado

Updated: Jun 23, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Requisitos estructurales para la función de un replicador cromosómico de levadura.

S Kearsey

    Cell
    |May 1, 1984
    PubMed
    Resumen

    Los investigadores identificaron una región central crucial de 14 bp esencial para la replicación autónoma en la levadura. Esta región, cerca del gen HO de Saccharomyces cerevisiae, es vital para la función de origen de la replicación del ADN.

    Área de la Ciencia:

    • Biología Molecular Biología Molecular
    • Genética de la levadura Genética de la levadura
    • Replicación del ADN Replicación del ADN

    Sus antecedentes:

    • El gen HO de Saccharomyces cerevisiae está asociado con una secuencia que permite la replicación autónoma en la levadura.
    • Comprender los requisitos estructurales de los orígenes de la replicación es clave para comprender los mecanismos de replicación del ADN.

    Objetivo del estudio:

    • Identificar los elementos estructurales críticos de un supuesto origen de replicación de levadura.
    • Para determinar la importancia funcional de secuencias específicas de ADN para la replicación autónoma in vivo.

    Principales métodos:

    • La mutagénesis por deleción se utilizó para eliminar segmentos de la secuencia de ADN.
    • Se empleó la mutagénesis puntual para alterar nucleótidos específicos dentro de la secuencia.

    Más Videos Relacionados

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
    08:40

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

    Published on: October 21, 2022

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
    07:48

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

    Published on: October 11, 2022

    Videos de Experimentos Relacionados

    Last Updated: Jun 23, 2026

    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
    06:40

    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

    Published on: March 22, 2018

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
    08:40

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

    Published on: October 21, 2022

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
    07:48

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

    Published on: October 11, 2022

  • El análisis funcional evaluó la replicación autónoma en la levadura.

    • Principales resultados:

    • Se identificó una región central de 14 bp como esencial para la replicación autónoma.
    • Las mutaciones puntuales dentro de esta región central abolieron la replicación.
    • Una secuencia flanqueante de 20 bp mejoró la eficiencia de la replicación, pero no fue estrictamente esencial.

    Conclusiones:

    • La secuencia del núcleo de 14 bp es un determinante crítico de la función de origen de replicación autónoma en la levadura.
    • Los elementos de secuencia que flanquean la región central contribuyen a la eficiencia de la replicación, pero no son necesarios para ella.