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Una secuencia corta de nucleótidos requerida para la regulación de HIS4 por el sistema general de control de la
Cell
|January 1, 1983
Resumen
Investigando el gen HIS4.
Área de la Ciencia:
- Biología molecular La biología molecular.
- Genética de la levadura genética de la levadura.
Sus antecedentes:
- El gen HIS4 en la levadura es crucial para la biosíntesis de la histidina.
- Comprender su regulación génica proporciona información sobre el control metabólico.
Objetivo del estudio:
- Identificar los elementos regulatorios dentro de la región no codificada HIS4 5' responsable de su control.
- Para aclarar el mecanismo del control general de la biosíntesis de aminoácidos en la expresión HIS4.
Principales métodos:
- Deleciones in vitro de la región no codificante HIS4 5'.
- La introducción de deleciones en el genoma de la levadura a través de la transformación.
- Análisis de los niveles de ARNm HIS4 en diferentes condiciones.
Principales resultados:
- Las supresiones hasta -235 no tuvieron ningún efecto en las funciones promotoras o reguladoras de HIS4.
- Las supresiones que se extienden a -138 afectaron la función promotora pero mantuvieron la regulación.
- Una supresión en -136 abolió la desrepresión, eliminando la secuencia 5'-TGACTC-3'.
- La reintroducción de esta secuencia restauró la respuesta reguladora.
Conclusiones:
- La secuencia 5'-TGACTC-3' es esencial para el control general de la biosíntesis de aminoácidos en HIS4.4.
- La expresión génica HIS4 parece estar bajo el control positivo de este elemento regulador.
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