Video Experimental Relacionado
Updated: Jul 24, 2026
10:00
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
Regulación transcripcional y posttranscripcional de la expresión génica inducida por el interferón en células humanas
Cell
|October 1, 1984
Resumen
El tratamiento con interferón induce ARN mensajeros específicos (ARNm) en las células del neuroblastoma, con algunos genes que muestran un aumento de la transcripción y la acumulación estable de ARNm. La regulación post-transcripcional también contribuye a elevar los niveles de ARNm.
Área de la Ciencia:
- Biología molecular La biología molecular.
- Inmunología Inmunología.
- La neurociencia es la neurociencia.
Sus antecedentes:
- Las interferonas son moléculas de señalización cruciales en el sistema inmunológico.
- Comprender la regulación génica por los interferones es clave para sus aplicaciones terapéuticas.
- Las células del neuroblastoma proporcionan un modelo para estudiar las respuestas celulares al interferón.
Objetivo del estudio:
- Identificar y caracterizar los genes inducidos por el interferón en las células de neuroblastoma T98G.
- Investigar la regulación transcripcional y post-transcripcional de estos genes inducidos.
- Para entender la cinética de la acumulación de ARNm y los cambios en la expresión génica.
Principales métodos:
- La clonación de ADN complementario (ADNc) a partir de células tratadas con interferón.
- Análisis de la mancha del norte para detectar los niveles de ARNm.
- Análisis de las tasas de transcripción génica a lo largo del tiempo.
Principales resultados:
- Se identificaron dieciocho ADNc correspondientes a siete distintos ARNm inducidos por el interferón.
- Un ARNm inducido codifica para la metalotioneína II, otro para un HLA de clase I.
- La acumulación de ARNm continuó durante 1 día con el interferón, pero cesó tras su eliminación; los ARNm inducidos fueron estables.
- La transcripción génica se duplicó en 5 minutos y alcanzó su punto máximo a los 60 minutos, luego disminuyó, mientras que los niveles de ARNm continuaron aumentando, lo que indica una regulación post-transcripcional.
Conclusiones:
- El interferón induce la expresión de genes específicos en las células del neuroblastoma, incluidas las conocidas y las nuevas.
- Tanto la activación transcripcional como los mecanismos post-transcripcionales contribuyen a la acumulación sostenida de ARNm inducidos por el interferón.
- La estabilidad de los ARNm inducidos y la regulación compleja resaltan la intrincada respuesta celular al interferón.
Videos de Conceptos Relacionados
01:42What is Gene Expression?
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
01:36What is Gene Expression?
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
01:30Inhibitors of Viral Protein Synthesis
Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...