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Regulación específica de la célula del gen c-myc por mitógenos linfocíticos y factores de crecimiento derivados de
Cell
|December 1, 1983
Resumen
El gen c-myc es inducido por señales de crecimiento de una manera dependiente del ciclo celular. Sus niveles de ARN mensajero (ARNm) aumentan rápidamente sin la síntesis de nuevas proteínas, lo que sugiere la regulación por una proteína de corta duración.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Los oncogenes son oncogenes.
- Regulación del ciclo celular Reglamento del ciclo celular.
Sus antecedentes:
- El gen c-myc es un proto-oncogén crucial involucrado en la proliferación celular.
- Comprender la regulación c-myc es clave para descifrar el desarrollo del cáncer.
- Se sabe que los factores de crecimiento influyen en los procesos celulares, incluida la expresión génica.
Objetivo del estudio:
- Investigar la regulación de la expresión génica c-myc por señales específicas de crecimiento.
- Para determinar el papel de la progresión del ciclo celular en la inducción de c-myc.
- Para explorar la relación entre c-myc y otros oncogenes como c-sis.
Principales métodos:
- Tratamiento de linfocitos y fibroblastos con mitógenos como el lipopolisacárido, la concanavalina A y el factor de crecimiento derivado de plaquetas (PDGF).
- Medición de los niveles de c-myc mRNA utilizando técnicas sensibles a cambios rápidos en la expresión génica.
- Experimentos con cicloheximida para evaluar la necesidad de una nueva síntesis de proteínas.
Principales resultados:
- Las señales específicas de crecimiento (LPS, ConA, PDGF) inducen el ARNm c-mítico en linfocitos y fibroblastos, respectivamente.
- Los niveles de c-myc mRNA aumentan de 10 a 40 veces dentro de 1 a 3 horas de la adición de mitógeno.
- La inducción se produce en presencia de cicloheximida, lo que indica que no requiere una nueva síntesis de proteínas.
- El ARNm c-myc es "superinducido" por la cicloheximida y el mitógeno, lo que sugiere una regulación por una proteína lábil.
Conclusiones:
- c-myc es un gen inducible regulado por señales de crecimiento de una manera dependiente del ciclo celular.
- El mecanismo de inducción no requiere la síntesis de proteínas de novo, lo que apunta a un control post-transcripcional o de traslación rápida.
- Es probable que una proteína reguladora lábil controle los niveles de c-myc.
- Existe un vínculo regulador entre c-myc y c-sis (gen PDGF).
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