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RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Cooperative Binding of Transcription Regulators02:13

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Co-activators and Co-repressors02:04

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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

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Updated: Jun 23, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Published on: September 20, 2018

Cambiar la especificidad de unión al ADN de un represor.

P Youderian, A Vershon, S Bouvier

    Cell
    |December 1, 1983
    PubMed
    Resumen

    Los investigadores identificaron mutaciones específicas en la proteína represora de Mnt que alteran su especificidad de unión al ADN. Este represor P22 del fago de Salmonella ahora se une a un operador mutado más fuertemente que el original, lo que demuestra un cambio clave en su reconocimiento genético.

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    Published on: May 5, 2023

    Área de la Ciencia:

    • Biología Molecular Biología Molecular
    • Genética La genética.
    • Virología Virología.

    Sus antecedentes:

    • El represor Mnt del fago de Salmonella P22 es crucial para regular la expresión génica al unirse a una secuencia de ADN operador específica.
    • La comprensión de las interacciones represor-operador es clave para descifrar los mecanismos de control genético viral.

    Objetivo del estudio:

    • Para investigar cómo mutaciones específicas en el represor Mnt afectan su especificidad de unión al ADN.
    • Para identificar los cambios en los aminoácidos responsables de la alterada afinidad y especificidad de unión.

    Principales métodos:

    • Construcción de un sitio de unión Mnt mutante con mutaciones simétricas constitutivas del operador.
    • Selección de mutantes represores de Mnt con preferencias de unión alteradas.
    • Ensayos de unión al ADN in vitro utilizando proteínas Mnt purificadas de tipo silvestre y mutantes.

    Principales resultados:

    • Las mutaciones en el codón CAC del represor Mnt (His6 a Pro) dieron como resultado un cambio significativo en la especificidad de unión al ADN.
    • El Mnt de tipo salvaje se une fuertemente al operador de tipo salvaje, pero el operador mutante tiene una afinidad 1000 veces menor.
    • Las proteínas Mnt mutantes exhibieron afinidades de unión invertidas, favoreciendo al operador mutante.

    Conclusiones:

    • La sustitución de His6 a Pro en el represor Mnt es crítica para su especificidad de unión al ADN.
    • Este estudio demuestra cómo las mutaciones dirigidas pueden reprogramar las interacciones entre el ADN represor y el ADN.
    • Los hallazgos proporcionan información sobre la base molecular del reconocimiento de secuencias en los represores de fagos.