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Videos de Conceptos Relacionados

Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
Transcription01:17

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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Video Experimental Relacionado

Updated: Jul 17, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Las transcripciones reguladas durante el desarrollo embrionario normal y la transformación oncogénica comparten un

D Murphy, P M Brickell, D S Latchman

    Cell
    |December 1, 1983
    PubMed
    Resumen

    El conjunto 1 de elementos repetitivos identifica transcripciones reguladas en el desarrollo en embriones de ratón. Su abundancia disminuye al diferenciarse en líneas celulares pluripotentes, excepto en las células F9.

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    09:58

    Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

    Published on: June 27, 2020

    Área de la Ciencia:

    • Biología Molecular Biología Molecular
    • Biología del desarrollo Biología del desarrollo.
    • La genómica es la genómica.

    Sus antecedentes:

    • Clones de ADNc previamente aislados homólogos al ARNm elevado en fibroblastos de ratón transformados.
    • El conjunto 1 de clones contiene un elemento repetitivo disperso que se encuentra miles de veces en el genoma del ratón.

    Objetivo del estudio:

    • Investigar el papel de los elementos repetitivos del conjunto 1 en la regulación génica durante el desarrollo del ratón.
    • Para analizar los patrones de expresión de transcripciones relacionadas con el conjunto 1 en modelos embrionarios y de líneas celulares.

    Principales métodos:

    • Aislamiento de clones de ADNc homólogos a ARNm específicos.
    • Análisis de la distribución de elementos repetitivos en el genoma del ratón.
    • Cuantificación de las transcripciones de ARN utilizando técnicas de hibridación.
    • Comparación de patrones de transcripción en tejidos embrionarios y líneas celulares (EC, EK, F9).

    Principales resultados:

    • La repetición del conjunto 1 identifica numerosas transcripciones reguladas cuantitativamente en embriones de ratón, que constituyen el 1-3% del ARN poliadenilado en la expresión máxima.
    • Un patrón de transcripción similar al de los embriones de gestación media se observa en las líneas celulares pluripotenciales EC y EK.
    • La abundancia de ARN disminuye con la diferenciación in vitro de las células EC y EK.
    • La línea celular F9 EC, con capacidad de desarrollo restringida, muestra un patrón de transcripción de Set 1 más simple.

    Conclusiones:

    • Los elementos repetitivos del conjunto 1 están asociados con transcripciones reguladas en el desarrollo en ratones.
    • La expresión de estas transcripciones está vinculada a procesos de pluripotencia y diferenciación.
    • Las variaciones en los patrones de transcripción del conjunto 1 pueden reflejar diferencias en el potencial de desarrollo de las líneas celulares.