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El butirato de sodio inhibe la desacetilación de histonas en las células de cultivo
Cell
|May 1, 1978
Resumen
El butirato de sodio aumenta la acetilación de la histona al inhibir las desacetilasas de la histona. Este ácido graso afecta los niveles de histona en varias líneas celulares, afectando la expresión génica.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- La acetilación de la histona regula la expresión génica.
- Se sabe que el butirato de sodio afecta los procesos celulares.
Objetivo del estudio:
- Investigar el mecanismo por el cual el butirato de sodio influye en la acetilación de la histona.
- Para determinar si el butirato afecta las tasas de acetilación o desacetilación de la histona.
Principales métodos:
- Tratamiento de varias líneas celulares de vertebrados con butirato de sodio.
- Análisis de los niveles de acetilación de histonas (H3, H4, H2A, H2B).
- Análisis de la actividad de la histona desacetilasa en extractos libres de células.
Principales resultados:
- El butirato de sodio causó hiperacetilación de las histonas H3 y H4 en todas las líneas celulares probadas.
- Las histonas H2A y H2B también se vieron afectadas en las células IRC8 de la rata.
- El butirato inhibió la desacetilación de histonas en extractos libres de células, no la acetilación.
- La actividad de la histona desacetilasa fue inhibida por el butirato in vitro.
Conclusiones:
- El butirato de sodio actúa como un inhibidor de las histonas desacetilasas (HDAC).
- Esta inhibición ocurre tanto in vivo como in vitro.
- El efecto del butirato sobre la acetilación de la histona está mediado por la supresión de la desacetilación.
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