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La secuencia transformadora HL-60: un oncogén ras que coexiste con genes myc alterados en tumores hematopoyéticos
Cell
|July 1, 1983
Resumen
Los investigadores identificaron un nuevo oncogén, N-ras, de las células de leucemia humana. Su pérdida revirtió la transformación de las células cancerosas, lo que sugiere su papel crítico en el desarrollo del tumor y su relación con otros tipos de cáncer.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Oncología Oncología.
- Genética La genética.
Sus antecedentes:
- La línea celular de la leucemia promyelocítica humana HL-60 contiene un oncogén transformador.
- Los oncogenes son impulsores clave de la transformación celular y el desarrollo del cáncer.
Objetivo del estudio:
- Identificar y caracterizar el oncogén responsable de la transformación en la línea celular HL-60.
- Investigar la relación entre el oncogén HL-60 y los oncogenes encontrados en otros tumores humanos y los oncogenes virales.
Principales métodos:
- Transfección en serie de fibroblastos de ratón NIH / 3T3 con el oncogén HL-60.
- La clonación molecular y la preparación de sondas específicas de oncogenes.
- Análisis de las células transfectadas para la reversión morfológica y la expresión oncogénica.
Principales resultados:
- El oncogén transfectado, denominado N-ras, indujo la transformación en las células NIH / 3T3.
- Pérdida del oncogén N-ras transfectado correlacionado con la reversión a un fenotipo no transformado.
- Los N-ras mostraron una estrecha homología con los oncogenes del linfoma de Burkitt, la leucemia mielógena aguda, el adenocarcinoma del colon, el neuroblastoma y los sarcomas.
- N-ras está distantemente relacionado con los oncogenes del virus del sarcoma de Kirsten y Harvey.
- El N-ras activo coexiste con oncogenes micológicos alterados en los tumores HL-60 y AW Ramos.
Conclusiones:
- El oncogén N-ras juega un papel crítico en la transformación de las células leucémicas HL-60.
- N-ras representa un gen transformador común a través de varias neoplasias humanas.
- Los hallazgos sugieren un mecanismo de tumorigénesis en múltiples etapas que involucra tanto a los oncogenes ras como a los myc.
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