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El factor de crecimiento epidérmico estimula rápidamente la transcripción del gen de la prolactina
Nature
|November 11, 1982
Resumen
El factor de crecimiento epidérmico (EGF) estimula rápidamente la transcripción del gen de la prolactina en las células GH4, aumentando la síntesis de prolactina. Este efecto transcripcional temprano puede mediar respuestas celulares posteriores al EGF.
Área de la Ciencia:
- Biología celular Biología celular.
- Endocrinología Molecular La Endocrinología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- El factor de crecimiento epidérmico (EGF) es un factor de crecimiento conocido que afecta a varios procesos celulares.
- El FEAG influye en la glucosa, la absorción de aminoácidos y la síntesis de proteínas, ARN y ADN.
- Las primeras respuestas del FEAG incluyen flujos de sodio y fosforilación de tirosina, potencialmente mediando sus efectos.
Objetivo del estudio:
- Investigar los efectos del FEAG en la síntesis de prolactina en las células pituitarias de la rata GH4.
- Aclarar el mecanismo detrás de las acciones del FEAG en las funciones celulares.
- Determinar si el FEAG afecta la transcripción del gen de la prolactina.
Principales métodos:
- Utilizó la línea celular pituitaria GH4 de la rata.
- El FEAG administrado a cultivos celulares.
- Se midió la síntesis de prolactina y el crecimiento celular.
- Tasas de transcripción de genes de prolactina evaluadas.
Principales resultados:
- La adición del FEAG estimuló la síntesis de prolactina de tres a seis veces.
- El EGF inhibió parcialmente el crecimiento celular GH4.
- El aumento de la síntesis de prolactina se debió a la rápida estimulación de la transcripción del gen de la prolactina por el FEAG.
Conclusiones:
- El FEG estimula rápidamente la transcripción del gen de la prolactina en las células GH4.
- Esta estimulación transcripcional conduce a un aumento en la síntesis de prolactina.
- Los efectos transcripcionales tempranos del FEAG pueden ser la base de sus funciones más amplias en la regulación del ciclo celular.
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