Video Experimental Relacionado

Updated: May 5, 2026

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Adhesión celular del teratocarcinoma: identificación de una proteína de la superficie celular involucrada en la

C Yoshida, M Takeichi

|February 1, 1982

Resumen

Las células de teratocarcinoma poseen un sitio único de adhesión celular dependiente de calcio (t-CDS). Se ha identificado que un fragmento de proteína específico (p140) es crucial para la función del t-CDS y la formación de la monocapa de células de teratocarcinoma.

Área de la Ciencia:

Biología celular Biología celular.
Biología del desarrollo Biología del desarrollo.
La bioquímica es la bioquímica.

Sus antecedentes:

Las células de teratocarcinoma exhiben una adhesión célula-célula dependiente de calcio mediada por un sitio único (t-CDS).
Este t-CDS es inactivado de manera única por la tripsina en ausencia de iones de calcio (Ca2+).

Objetivo del estudio:

Para identificar los componentes moleculares responsables de la adhesión celular dependiente del calcio en las células de teratocarcinoma.
Caracterizar el papel de los antígenos específicos de la superficie celular en la mediación de la agregación celular del teratocarcinoma y la formación de monocapas.

Principales métodos:

Utilizados fragmentos Fab de anticuerpos contra las células de teratocarcinoma F9 tratadas con tripsina y calcio (anti-TC-F9) para inhibir la agregación celular.
Realizó experimentos de absorción con células F9 tratadas (TC-F9 y TE-F9) para identificar antígenos específicos.
Empleó inmunoprecipitación y análisis de peso molecular para caracterizar los componentes de la superficie celular.
Investigó el efecto de una sustancia liberada (p34) en la unión de anticuerpos y la disociación celular.

Principales resultados:

Los fragmentos Anti-TC-F9 Fab inhiben la agregación celular del teratocarcinoma mediada por el t-CDS.
Un componente específico, p140 (peso molecular ~140.000), fue identificado en las células TC-F9 y es reconocido por el anti-TC-F9.9.
Un fragmento tríptico, p34 (peso molecular ~34,000), liberado de las células TC-F9, neutralizó el efecto inhibidor de anti-TC-F9 Fab e interfirió con la inmunoprecipitación p140.
Anti-TC-F9 Fab indujo la disociación de las monocapas de células de teratocarcinoma, un efecto neutralizado por p34.

Conclusiones:

El componente p140 es esencial para la función del sitio de adhesión celular dependiente de calcio (t-CDS) en las células de teratocarcinoma.
p140 se identifica como una molécula de adhesión celular crítica para el establecimiento de monocapas celulares de teratocarcinoma.
p34 representa un fragmento tríptico de p140, apoyando aún más el papel de p140 en la adhesión celular.

Más Videos Relacionados

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

Videos de Conceptos Relacionados

Overview of Cell-Matrix Interactions

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

Structure of Cadherins

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...

Cadherins in Tissue Organization

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...

Cell Adhesion Molecules - Types and Functions

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...