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Permeabilidad de las uniones de hueco en el borde segmental en la epidermis de insectos
Cell
|February 1, 1982
Resumen
La comunicación célula-célula en la epidermis de los insectos es específica del segmento. Las uniones de la brecha en las fronteras segmentales pueden tener una permeabilidad única, lo que restringe la transferencia de colorante entre los compartimentos de desarrollo en insectos de las algas lecheras y moscas voladoras.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- Fisiología de los insectos.
Sus antecedentes:
- La comunicación célula-célula es crucial para el desarrollo del tejido.
- Las fronteras segmentales definen los compartimentos de desarrollo en los insectos.
- Las uniones gap median la comunicación intercelular.
Objetivo del estudio:
- Para investigar los patrones de comunicación célula-célula a través de las fronteras segmentales en la epidermis de los insectos.
- Para determinar si las uniones de brecha restringen la comunicación entre los compartimentos de desarrollo.
- Para comparar la comunicación en el insecto de las algas lecheras (Oncopeltus fasciatus) y la mosca del soplo (Calliphora erythrocephala).
Principales métodos:
- Pruebas de acoplamiento iónico para evaluar la comunicación eléctrica.
- Microinyección de tintes fluorescentes (amarillo Lucifer) y trazadores aniónicos (EDTA de plomo).
- Mapeo de los patrones de transferencia de colorantes en la epidermis larval de Oncopeltus fasciatus y Calliphora erythrocephala.
Principales resultados:
- Las células epidérmicas estaban acopladas iónicamente dentro de segmentos en ambas especies.
- La transferencia de Lucifer Yellow estaba restringida a células dentro del mismo segmento, no cruzando fronteras segmentales.
- El plomo-EDTA cruzó libremente las fronteras segmentales en Calliphora, lo que indica una permeabilidad diferencial.
- Fallo de la transferencia amarilla de Lucifer sugiere propiedades alteradas de unión de brecha en las fronteras segmentales.
Conclusiones:
- Las células epidérmicas de los insectos se comunican de manera diferente a través de las fronteras segmentarias en comparación con dentro de los segmentos.
- Las uniones de brechas en las fronteras segmentales exhiben características de permeabilidad distintas.
- Esto sugiere un papel para la permeabilidad selectiva en el mantenimiento de la compartimentación del desarrollo.
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