Video Experimental Relacionado
Updated: May 10, 2026
11:17
Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor
Published on: February 10, 2014
Cambios inducidos por promotores tumorales en la permeabilidad de las uniones estrechas de las células epiteliales
Cell
|January 1, 1981
Resumen
El promotor tumoral 12-O-tetradecanoilforbol-13-acetato (TPA) aumenta la permeabilidad de las células renales y altera la forma de las células. TPA TPA TPA es el nombre que se le da a las
Área de la Ciencia:
- Biología celular Biología celular.
- Biología epitelial Biología epitelial.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Se sabe que el promotor tumoral 12-O-tetradecanoilforbol-13-acetato (TPA) induce varias respuestas celulares.
- Las uniones estrechas epiteliales regulan el transporte paracelular y mantienen la integridad del tejido.
- Comprender los efectos del TPA en las células epiteliales es crucial para la investigación del cáncer.
Objetivo del estudio:
- Investigar los efectos del TPA en la permeabilidad y morfología de las células epiteliales renales (MDCK).
- Para determinar el papel del activador del plasminógeno (AP) en los cambios inducidos por el TPA.
- Examinar la participación de los componentes del citoesqueleto en la acción del TPA.
Principales métodos:
- Tratamiento de las células MDCK con TPA.
- Medición de la permeabilidad transepitelial.
- Análisis de la morfología celular usando microscopía.
- Evaluación de la síntesis del activador del plasminógeno (AP).
- La inhibición de la síntesis de AP y la función citoesquelética con fármacos específicos.
Principales resultados:
- El TPA aumentó significativamente la permeabilidad de las uniones estrechas y alteró la morfología celular en 2-4 horas.
- El TPA estimuló un aumento de 10 a 20 veces en la síntesis y secreción de PA.
- La inhibición de la síntesis de PA no impidió los cambios morfológicos inducidos por TPA o el aumento de la permeabilidad.
- Los fármacos que inhiben la función citoesquelética impiden cambios morfológicos, pero no aumentan la permeabilidad.
Conclusiones:
- Los efectos del TPA en la permeabilidad de las uniones estrechas y la morfología celular son complejos y se producen tempranamente.
- El activador del plasminógeno (AP) no está involucrado en los cambios inducidos por el TPA temprano en las uniones cerradas o la morfología celular.
- El TPA puede actuar directamente sobre las uniones estrechas epiteliales y los componentes del citoesqueleto.
Más Videos Relacionados
10:22In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
Published on: October 19, 2018
09:42Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
Published on: June 26, 2019
Videos de Conceptos Relacionados
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
02:17The Tumor Microenvironment
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
02:30Metastasis
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
01:12Loss of Tumor Suppressor Gene Functions
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
01:13Cell-matrix's Response to Mechanical Forces
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
01:29Tight Junctions
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...