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Funciones superficiales durante la Mitosis I: fagocitosis, pinocitosis y movilidad de la Con A ligada a la superficie
Cell
|October 1, 1978
Resumen
La endocitosis celular, incluida la fagocitosis y la pinocitosis, se inhibe significativamente durante la mitosis. Esta inhibición afecta la captación de partículas y los procesos de fase fluida, impactando la señalización celular y las propiedades de las células recién formadas.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Los procesos celulares como la fagocitosis y la pinocitosis son cruciales para la absorción y señalización de nutrientes.
- La mitosis implica una reorganización celular significativa, que puede afectar la dinámica de la membrana y las vías endocíticas.
Objetivo del estudio:
- Investigar el impacto de la mitosis en la fagocitosis, la pinocitosis y la distribución superficial de la concanavalina A (ConA) en las células de los mamíferos.
- Para correlacionar las fases mitóticas específicas con las alteraciones en la actividad endocítica y la membrana ligada ConA.
Principales métodos:
- Utilizado Hoechst 33258 colorante para la tinción de cromosomas para identificar las fases mitóticas.
- Se evaluó la fagocitosis utilizando partículas opsonizadas (eritrocitos recubiertos con IgG) y no opsonizadas (perlas de látex).
- Se midió la pinocitosis fluida a través de la captación de la peroxidasa del rábano picante y la pinocitosis adsortiva de ConA utilizando el etiquetado fluorescente.
Principales resultados:
- La fagocitosis de las partículas opsonizadas y no opsonizadas se reduce notablemente durante la mitosis.
- La pinocitosis fluida se inhibe desde la profase media a través de la telofase en las células J774 y CHO.
- La internalización de la concanavalina A (ConA) se suprime, con ConA restringido a la membrana plasmática durante la mitosis y se acumula en el surco de la escisión.
Conclusiones:
- Los procesos endocíticos se inhiben significativamente durante la mitosis celular de los mamíferos.
- Esta inhibición de la endocitosis puede desempeñar un papel en la regulación de las actividades celulares durante la mitosis e influir en las características de las células hijas.
- La redistribución de ConA observada pone de relieve la reorganización dinámica de la membrana celular durante la división celular.
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